Team:Freiburg/Notebook/13 July

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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 13 July </a>
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==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==
Line 18: Line 33:
|DNA concentration
|DNA concentration
|260/280
|260/280
-
|260|230
+
|260/230
|-
|-
|cph8a
|cph8a
Line 50: Line 65:
|1.81
|1.81
|}
|}
-
 
===Testdigest===
===Testdigest===
Line 60: Line 74:
*Enzyme 1: EcoRI
*Enzyme 1: EcoRI
*Enzyme 2: PstI
*Enzyme 2: PstI
 +
<br/>
 +
<br/>
 +
===Transformation===
 +
 +
'''Investigators: Julia'''
 +
 +
Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.
 +
 +
<span style="color:blue;">Comments: No colonies grew on the plates.</span>
 +
 +
 +
===PCR===
 +
 +
'''Investigators: Julia'''
 +
 +
New PCR for red light receptor.
 +
 +
Primer:
 +
*P10
 +
*P11
 +
 +
DNA template:
 +
*M30109
 +
 +
<span style="color:blue;">Comments: There were no bands on the gel.</span>
 +
<br/>
 +
<br/>
==<span style="color:grey;">Precipitator</span>==
==<span style="color:grey;">Precipitator</span>==
Line 70: Line 111:
<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span>
<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span>
-
Miniprep of the plastik-binding domain:
+
Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).
{| cellpadding="10" cellspacing="0" border="1"
{| cellpadding="10" cellspacing="0" border="1"
-
|samples
+
|sample / sample*
|DNA concentration
|DNA concentration
|280/230
|280/230
|260/230
|260/230
|-
|-
-
|M2a alpha/M2a* alpha
+
|M2a alpha / M2a* alpha
-
|66.5/91.6
+
|66.5 / 91.6
-
|1.84/1.75
+
|1.84 / 1.75
-
|1.76/1.40
+
|1.76 / 1.40
|-
|-
-
|M2a beta/M2a* beta
+
|M2a beta / M2a* beta
-
|83.8/13.8
+
|83.8 / 13.8
-
|1.80/1.72
+
|1.80 / 1.72
-
|1.52/0.97
+
|1.52 / 0.97
|-
|-
-
|M2a gamma/M2a* gamma
+
|M2a gamma / M2a* gamma
-
|118.9/56.1
+
|118.9 / 56.1
-
|1.68/1.86
+
|1.68 / 1.86
-
|1.00/2.13
+
|1.00 / 2.13
|-
|-
-
|M2a delta/M2a* delta
+
|M2a delta / M2a* delta
-
|101.5/66.0
+
|101.5 / 66.0
-
|1.74/1.85
+
|1.74 / 1.85
-
|1.26/2.16
+
|1.26 / 2.16
|-
|-
-
|M2a epsilon/M2a* epsilon
+
|M2a epsilon / M2a* epsilon
-
|112.9/69.9
+
|112.9 / 69.9
-
|1.68/1.82
+
|1.68 / 1.82
-
|1.00/1.82
+
|1.00 / 1.82
|-
|-
-
|M2b alpha/M2a* alpha
+
|M2b alpha / M2a* alpha
-
|79.7/74.9
+
|79.7 / 74.9
-
|1.87/1.83
+
|1.87 / 1.83
-
|2.20/2.14
+
|2.20 / 2.14
|-
|-
|M2b Beta/M2b* beta
|M2b Beta/M2b* beta
Line 113: Line 154:
|1.04/2.18
|1.04/2.18
|-
|-
-
|M2b gamma/M2b* gamma
+
|M2b gamma / M2b* gamma
-
|111.2/72.0
+
|111.2 / 72.0
-
|1.70/1.83
+
|1.70 / 1.83
-
|1.08/1.99
+
|1.08 / 1.99
|-
|-
-
|M2b epsilon/M2b* epsilon
+
|M2b epsilon / M2b* epsilon
-
|88.5/90.09
+
|88.5 / 90.09
-
|1.72/1.79
+
|1.72 / 1.79
-
|1.19/1.78
+
|1.19 / 1.78
|-
|-
-
|M3 alpha/M3* alpha
+
|M3 alpha / M3* alpha
-
|136.3/93.9
+
|136.3 / 93.9
-
|1.71/1.78
+
|1.71 / 1.78
-
|1.10/1.74
+
|1.10 / 1.74
|-
|-
-
|M3 beta/M3* beta
+
|M3 beta / M3* beta
-
|54.7/79.2
+
|54.7 / 79.2
-
|1.91/1.83
+
|1.91 / 1.83
-
|2.03/2.13
+
|2.03 / 2.13
|-
|-
-
|M3 gamma/M3* gamma
+
|M3 gamma / M3* gamma
-
|130.6/141.7
+
|130.6 / 141.7
-
|1.66/1.80
+
|1.66 / 1.80
-
|0.87/1.88
+
|0.87 / 1.88
|-
|-
-
|M3 delta/M3* delta
+
|M3 delta / M3* delta
-
|79.6/82.9
+
|79.6 / 82.9
-
|1.76/1.85
+
|1.76 / 1.85
-
|1.27/2.19
+
|1.27 / 2.19
|-
|-
-
|M3 epsilon/M3* epsilon
+
|M3 epsilon / M3* epsilon
-
|84.1/122.5
+
|84.1 / 122.5
-
|1.79/1.69
+
|1.79 / 1.69
-
|1.45/1.03
+
|1.45 / 1.03
|}
|}
 +
<br/>
 +
<br/>
 +
 +
===Testdigest===
 +
 +
'''Investigators: Sophie'''
 +
 +
The minipreps were digested and loaded onto a gel.
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:55, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples DNA concentration 260/280 260/230
cph8a 33.7 1.79 1.74
cph8b 41.2 1.79 1.39
cph8c 24.1 1.87 1.47
cph8d 30 1.74 1.84
cph8e 34 1.81 1.86
cph8f 30 1.83 1.81

Testdigest

Investigators: Julia

Testdigest of cph8

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI



Transformation

Investigators: Julia

Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.

Comments: No colonies grew on the plates.


PCR

Investigators: Julia

New PCR for red light receptor.

Primer:

  • P10
  • P11

DNA template:

  • M30109

Comments: There were no bands on the gel.

Precipitator

Miniprep

Investigators: Sophie, Sandra

Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.

Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).

sample / sample* DNA concentration 280/230 260/230
M2a alpha / M2a* alpha 66.5 / 91.6 1.84 / 1.75 1.76 / 1.40
M2a beta / M2a* beta 83.8 / 13.8 1.80 / 1.72 1.52 / 0.97
M2a gamma / M2a* gamma 118.9 / 56.1 1.68 / 1.86 1.00 / 2.13
M2a delta / M2a* delta 101.5 / 66.0 1.74 / 1.85 1.26 / 2.16
M2a epsilon / M2a* epsilon 112.9 / 69.9 1.68 / 1.82 1.00 / 1.82
M2b alpha / M2a* alpha 79.7 / 74.9 1.87 / 1.83 2.20 / 2.14
M2b Beta/M2b* beta 122.3/74.4 1.71/1.84 1.04/2.18
M2b gamma / M2b* gamma 111.2 / 72.0 1.70 / 1.83 1.08 / 1.99
M2b epsilon / M2b* epsilon 88.5 / 90.09 1.72 / 1.79 1.19 / 1.78
M3 alpha / M3* alpha 136.3 / 93.9 1.71 / 1.78 1.10 / 1.74
M3 beta / M3* beta 54.7 / 79.2 1.91 / 1.83 2.03 / 2.13
M3 gamma / M3* gamma 130.6 / 141.7 1.66 / 1.80 0.87 / 1.88
M3 delta / M3* delta 79.6 / 82.9 1.76 / 1.85 1.27 / 2.19
M3 epsilon / M3* epsilon 84.1 / 122.5 1.79 / 1.69 1.45 / 1.03



Testdigest

Investigators: Sophie

The minipreps were digested and loaded onto a gel.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/13_July"