Team:Freiburg/Notebook/13 July

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Waiting for sequencing results of parts BBa_15008 and BBa_15009.
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<a href="https://2011.igem.org/Team:Freiburg/Notebook/12_July">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 13 July </a>
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==<span style="color:red;">red light receptor</span>==
 +
 
 +
'''Investigators: Julia'''
 +
 
 +
Waiting for sequencing results of parts BBa_I15008 and BBa_I15009.
Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3.
Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3.
She also did a test digest to see if the insert is in the vector.
She also did a test digest to see if the insert is in the vector.
 +
 +
===Miniprep===
 +
 +
'''Investigators: Jakob'''
 +
 +
Miniprep of cph8 from M30109 in pSB1T3
 +
DNA concentration was measured with NanoDrop:
 +
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|samples
 +
|DNA concentration
 +
|260/280
 +
|260/230
 +
|-
 +
|cph8a
 +
|33.7
 +
|1.79
 +
|1.74
 +
|-
 +
|cph8b
 +
|41.2
 +
|1.79
 +
|1.39
 +
|-
 +
|cph8c
 +
|24.1
 +
|1.87
 +
|1.47
 +
|-
 +
|cph8d
 +
|30
 +
|1.74
 +
|1.84
 +
|-
 +
|cph8e
 +
|34
 +
|1.81
 +
|1.86
 +
|-
 +
|cph8f
 +
|30
 +
|1.83
 +
|1.81
 +
|}
 +
 +
===Testdigest===
 +
 +
'''Investigators: Julia'''
 +
 +
Testdigest of cph8
 +
 +
*Enzyme 1: EcoRI
 +
*Enzyme 2: PstI
 +
<br/>
 +
<br/>
 +
 +
===Transformation===
 +
 +
'''Investigators: Julia'''
 +
 +
Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.
 +
 +
<span style="color:blue;">Comments: No colonies grew on the plates.</span>
 +
 +
 +
===PCR===
 +
 +
'''Investigators: Julia'''
 +
 +
New PCR for red light receptor.
 +
 +
Primer:
 +
*P10
 +
*P11
 +
 +
DNA template:
 +
*M30109
 +
 +
<span style="color:blue;">Comments: There were no bands on the gel.</span>
 +
<br/>
 +
<br/>
 +
 +
==<span style="color:grey;">Precipitator</span>==
 +
 +
===Miniprep===
 +
 +
'''Investigators: Sophie, Sandra'''
 +
 +
<span style="color:blue;">Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.</span>
 +
 +
Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).
 +
 +
{| cellpadding="10" cellspacing="0" border="1"
 +
|sample / sample*
 +
|DNA concentration
 +
|280/230
 +
|260/230
 +
|-
 +
|M2a alpha / M2a* alpha
 +
|66.5 / 91.6
 +
|1.84 / 1.75
 +
|1.76 / 1.40
 +
|-
 +
|M2a beta / M2a* beta
 +
|83.8 / 13.8
 +
|1.80 / 1.72
 +
|1.52 / 0.97
 +
|-
 +
|M2a gamma / M2a* gamma
 +
|118.9 / 56.1
 +
|1.68 / 1.86
 +
|1.00 / 2.13
 +
|-
 +
|M2a delta / M2a* delta
 +
|101.5 / 66.0
 +
|1.74 / 1.85
 +
|1.26 / 2.16
 +
|-
 +
|M2a epsilon / M2a* epsilon
 +
|112.9 / 69.9
 +
|1.68 / 1.82
 +
|1.00 / 1.82
 +
|-
 +
|M2b alpha / M2a* alpha
 +
|79.7 / 74.9
 +
|1.87 / 1.83
 +
|2.20 / 2.14
 +
|-
 +
|M2b Beta/M2b* beta
 +
|122.3/74.4
 +
|1.71/1.84
 +
|1.04/2.18
 +
|-
 +
|M2b gamma / M2b* gamma
 +
|111.2 / 72.0
 +
|1.70 / 1.83
 +
|1.08 / 1.99
 +
|-
 +
|M2b epsilon / M2b* epsilon
 +
|88.5 / 90.09
 +
|1.72 / 1.79
 +
|1.19 / 1.78
 +
|-
 +
|M3 alpha / M3* alpha
 +
|136.3 / 93.9
 +
|1.71 / 1.78
 +
|1.10 / 1.74
 +
|-
 +
|M3 beta / M3* beta
 +
|54.7 / 79.2
 +
|1.91 / 1.83
 +
|2.03 / 2.13
 +
|-
 +
|M3 gamma / M3* gamma
 +
|130.6 / 141.7
 +
|1.66 / 1.80
 +
|0.87 / 1.88
 +
|-
 +
|M3 delta / M3* delta
 +
|79.6 / 82.9
 +
|1.76 / 1.85
 +
|1.27 / 2.19
 +
|-
 +
|M3 epsilon / M3* epsilon
 +
|84.1 / 122.5
 +
|1.79 / 1.69
 +
|1.45 / 1.03
 +
|}
 +
<br/>
 +
<br/>
 +
 +
===Testdigest===
 +
 +
'''Investigators: Sophie'''
 +
 +
The minipreps were digested and loaded onto a gel.
 +
{{:Team:Freiburg/Templates/footer}}

Latest revision as of 00:55, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

red light receptor

Investigators: Julia

Waiting for sequencing results of parts BBa_I15008 and BBa_I15009. Julia did Minipreps of single colonies with PCR-ampilfied Chp8 in pSB1T3. She also did a test digest to see if the insert is in the vector.

Miniprep

Investigators: Jakob

Miniprep of cph8 from M30109 in pSB1T3 DNA concentration was measured with NanoDrop:

samples DNA concentration 260/280 260/230
cph8a 33.7 1.79 1.74
cph8b 41.2 1.79 1.39
cph8c 24.1 1.87 1.47
cph8d 30 1.74 1.84
cph8e 34 1.81 1.86
cph8f 30 1.83 1.81

Testdigest

Investigators: Julia

Testdigest of cph8

  • Enzyme 1: EcoRI
  • Enzyme 2: PstI



Transformation

Investigators: Julia

Transformation of BBa_I15010 (in the vector pSB2K3) in cells. Sophie plated out the cells.

Comments: No colonies grew on the plates.


PCR

Investigators: Julia

New PCR for red light receptor.

Primer:

  • P10
  • P11

DNA template:

  • M30109

Comments: There were no bands on the gel.

Precipitator

Miniprep

Investigators: Sophie, Sandra

Comments: Ligation, transformation and picking of the clones was done before the miniprep was performed.

Miniprep of the plastik-binding domain: There were always picked two colonies of the same plate (sample and sample*).

sample / sample* DNA concentration 280/230 260/230
M2a alpha / M2a* alpha 66.5 / 91.6 1.84 / 1.75 1.76 / 1.40
M2a beta / M2a* beta 83.8 / 13.8 1.80 / 1.72 1.52 / 0.97
M2a gamma / M2a* gamma 118.9 / 56.1 1.68 / 1.86 1.00 / 2.13
M2a delta / M2a* delta 101.5 / 66.0 1.74 / 1.85 1.26 / 2.16
M2a epsilon / M2a* epsilon 112.9 / 69.9 1.68 / 1.82 1.00 / 1.82
M2b alpha / M2a* alpha 79.7 / 74.9 1.87 / 1.83 2.20 / 2.14
M2b Beta/M2b* beta 122.3/74.4 1.71/1.84 1.04/2.18
M2b gamma / M2b* gamma 111.2 / 72.0 1.70 / 1.83 1.08 / 1.99
M2b epsilon / M2b* epsilon 88.5 / 90.09 1.72 / 1.79 1.19 / 1.78
M3 alpha / M3* alpha 136.3 / 93.9 1.71 / 1.78 1.10 / 1.74
M3 beta / M3* beta 54.7 / 79.2 1.91 / 1.83 2.03 / 2.13
M3 gamma / M3* gamma 130.6 / 141.7 1.66 / 1.80 0.87 / 1.88
M3 delta / M3* delta 79.6 / 82.9 1.76 / 1.85 1.27 / 2.19
M3 epsilon / M3* epsilon 84.1 / 122.5 1.79 / 1.69 1.45 / 1.03



Testdigest

Investigators: Sophie

The minipreps were digested and loaded onto a gel.

                       

Retrieved from "http://2011.igem.org/Team:Freiburg/Notebook/13_July"