Team:ETH Zurich

From 2011.igem.org

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|style="font-size:2em; heigh 30px" class="overview"|Our Sponsors
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Revision as of 00:51, 22 September 2011

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Smoke Sensors
Design2.png
Sensor Design 1: Acetaldehyde Sensor


Alternative Xylene system.png
Sensor Design 2: Xylene Sensor
Abstract
SmoColi is a bacterio-quantifier of smoke concentration that can be used as a passive smoke detector. Cigarette smoke contains a lot of different toxic and carcinogenic substances, most of which are volatile, e.g. acetaldehyde or xylene. Therefore we can use them as an airborne information carrier. The SmoColi cells are immobilized in an agarose-filled microfluidic device. The test solution is fed to one end of a microfluidic channel, in which a concentration gradient is established by diffusion and synthetic cellular degradation. The cells are engineered to sense a certain molecule. Some sensors were integrated as found in nature, others had to by synthetically re-designed, e.g. the fungal acetaldehyde-responsive transactivator. The sensor is linked to a band-pass filter that drives GFP expression. This allows establishment of an input-concentration-dependent, moving fluorescent band displaying quantitative information of the input. Finally, if the input concentration exceeds the threshold of malignance, a quorum-sensing-based mCherry alarm system springs into action, turning the entire device red.
Data Page
Team
Overview of our system
Signal Processing by SmoColi
Overview of our system
Our Sponsors

ETHZ-BASF.png    ETHZ-BSSE.png    ETHZ-Lonza.png    ETHZ-Merck Serono.png   ETHZ-Novartis.png    ETHZ-Roche.png    ETHZ-Syngenta.png

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