Team:Freiburg/Notebook/2 September

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<a href="https://2011.igem.org/Team:Freiburg/Notebook/1_September">Previous entry</a>
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<a href="https://2011.igem.org/Team:Freiburg/Notebook"> 2 September </a>
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==<span style="color:green;">green light receptor</span>==
==<span style="color:green;">green light receptor</span>==
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1:2 not a has inserts too!
1:2 not a has inserts too!
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===Digestion: 2A-assembly===
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'''Investigators: Sophie'''
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Digestion of the PCR products Notgate, Lovtap for the 2A-assembly.
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===Ligation:2A-assembly===
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'''Investigators: Sandra'''
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Ligation of the digested parts in the already digested vector pSB1C3.
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Ligation at room temperature over night.
==<span style="color:red;">red light receptor</span>==
==<span style="color:red;">red light receptor</span>==
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==<span style="color:orange;">Lysis cassette</span>==
==<span style="color:orange;">Lysis cassette</span>==
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===NAME OF YOUR EXPERIMENT===
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===3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017===
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'''Investigators:NAME'''
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'''Investigators:Theo'''
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<br>
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Sequencing of Part S66 (modified Lysis genes K124017) showed that it is a DNA fragment from our other projects, so another miniprep was done and sent for sequencing.
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*Miniprep data were good:
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**Concentration = 220ng/mikrol
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**260/280 = 2.04
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**260/230 = 2.19
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<br>
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No gel was run to see if there is a band!
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<br>
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<br/>
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==<span style="color:grey;">Precipitator</span>==
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'''Rüdiger''' 02.-05.09.
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Trafo, colony picking & miniprep of ligations from 01.09.
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Nothing worked
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Another trafo done with same ligation. Found out that linearized C3 vector has no SpC1 restriction site.
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==<span style="color:grey;">Precipitator</span>==
 
===Trafo===
===Trafo===

Latest revision as of 00:27, 22 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!

Contents

green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME


blue light receptor

MIniprep

Investigators: Sophie, Sandra

Miniprep of new ligated parts in pSB1C3 vector.

After miniprep samples were digested and loaded onto a gel. Not c has inserts:-)

Freiburg2011 Lovtap.jpg

Freiburg2011Lovtap and greenlight.jpg

1:2 not a has inserts too!

Digestion: 2A-assembly

Investigators: Sophie

Digestion of the PCR products Notgate, Lovtap for the 2A-assembly.

Ligation:2A-assembly

Investigators: Sandra

Ligation of the digested parts in the already digested vector pSB1C3. Ligation at room temperature over night.

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME



Lysis cassette

3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017

Investigators:Theo
Sequencing of Part S66 (modified Lysis genes K124017) showed that it is a DNA fragment from our other projects, so another miniprep was done and sent for sequencing.

  • Miniprep data were good:
    • Concentration = 220ng/mikrol
    • 260/280 = 2.04
    • 260/230 = 2.19


No gel was run to see if there is a band!

Precipitator

Rüdiger 02.-05.09.


Trafo, colony picking & miniprep of ligations from 01.09.

Nothing worked

Another trafo done with same ligation. Found out that linearized C3 vector has no SpC1 restriction site.


Trafo

Investigators: Julia

Trafo of ligated parts:

  1. pGEX vector (Amp. resistance)
  2. pGEX vector (Amp. resistance)
  3. pGEX vector (Amp. resistance)
  4. pSB1C3
  5. pET Duet vector (Amp. resistance)
  6. pET Duet vector (Amp. resistance)
  7. pET Duet vector (Amp. resistance)
  8. pSB1C3
  9. pSB1C3
  10. pSB1C3

Miniprep

Investigators: Sophie

miniprep of GFP-pbd with IPTG-inducible promoter. The pellets were green!!! So we finally got both promoter and plastic binding domain into a vector! We can make experiments with the pbd now.

                       

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