Team:Freiburg/Notebook/3 September
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{{:Team:Freiburg/Templates/header}} | {{:Team:Freiburg/Templates/header}} | ||
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+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/2_September">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 3 September </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/4_September">Next entry</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
+ | |||
+ | ===Testdigestion of 2a-assembly=== | ||
+ | |||
+ | '''Investigators: Julia''' | ||
+ | <br/> | ||
+ | Continuing from 31 of Aug. [https://2011.igem.org/Team:Freiburg/Notebook/31_August]<br/> | ||
+ | Before Miniprep of 20 different colonies.<br/> | ||
+ | Each minipreped DNA-sample (3μl)was digested with EcoRI and PstI.<br/> | ||
+ | <br/> | ||
+ | Result:<br/> | ||
+ | <br/> | ||
+ | [[File:Freiburg11 09 03Testdigest 2A.Jpg|400px|400px]] | ||
+ | <br/> | ||
+ | |||
+ | |||
+ | |||
+ | the samples | ||
+ | |||
+ | 46b= ho1 with a RBS (BBa_B0034)<br/> | ||
+ | |||
+ | 50b= RBS(BBa_B0034)+pcyA+terminator<br/> | ||
+ | |||
+ | 64b= Promotor (BBa_J23110)+CcaS<br/> | ||
+ | |||
+ | 67b= Promotor (BBa_J23110)+CcaR<br/> | ||
+ | |||
+ | were send on [https://2011.igem.org/Team:Freiburg/Notebook/6_September 6.09] to GATC for sequencing | ||
===3A-assembly:Digestion=== | ===3A-assembly:Digestion=== | ||
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Digestion of: | Digestion of: | ||
- | InsertI: | + | '''InsertI:''' |
- | *64 | + | *64, CcaR |
- | *46 | + | *46, ho1 |
with EcorI and SpeI | with EcorI and SpeI | ||
- | InsertII: | + | '''InsertII:''' |
- | *67 | + | *67, CcaS |
- | *50 | + | *50, pcyA |
with XbaI and PstI | with XbaI and PstI | ||
+ | |||
+ | For this assembly we used the constructs from the 2A-assembly made on [https://2011.igem.org/Team:Freiburg/Notebook/31_August 31.08] | ||
+ | |||
+ | Incubation for 6 hours at 37°C. Vector pSB1C3 was already digested,<br/> it was just treated with antarctic phosphatase for one hour. | ||
+ | |||
+ | ===3A-assembly:Ligation=== | ||
+ | |||
+ | '''Investigators: Julia''' | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
Line 31: | Line 78: | ||
To do: Trafo | To do: Trafo | ||
+ | |||
+ | |||
+ | ===Transformation=== | ||
+ | with ♥C3 Lig. and Not C3 Lig | ||
+ | |||
+ | '''Investigator: Sophie''' | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== |
Latest revision as of 00:27, 22 September 2011