Team:Freiburg/Notebook/19 September

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green light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

blue light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

red light receptor

NAME OF YOUR EXPERIMENT

Investigators:NAME

Lysis cassette

The_little_hoped_for_experiment_IV

Investigators:Theo

I took the 3 cultures and prepared some tubes for them to test... I made measurements of the OD after about 18h of incubation at 30°C, put 1ml from every culture in an eppi (2 eppis for every culture) and incubated 3 of them at 30°C and 3 at 42°C. The results can be seen on our Project Results page or the Registry (Part BBa_K608351 and BBa_K608352).

After that, I sent the samples for sequencing hoping I would get the results the next day, but typical GATC decided to delay them until the 21st of September!!!

Precipitator

Ligation

Procedure

PCR tube:

total volume 20 μl

1. add H₂O (17 μl -X-Y-Z)
2. add 2 μl Ligase Buffer 10x
3. add Insert 1, Insert 2(when proceeding from 3A digestion use 2 μl of each)
4. add Vector (20ng needed. When proceeding from 3A digestion use 2 μl)
5. Add 1 μl T4-DNA Ligase
6. Incubate 10-30 min at room temperature
7. heat for 20 minutes at 80°C
8. store at -20°C or directly proceed to transformation

Documentation:

Why are you doing this experiment? Where are your parts stored? Name the parts for ligation etc.

NAME OF YOUR EXPERIMENT

Investigators: NAME