Team:Freiburg/Notebook/11 September
From 2011.igem.org
(Difference between revisions)
(9 intermediate revisions not shown) | |||
Line 1: | Line 1: | ||
{{:Team:Freiburg/Templates/header}} | {{:Team:Freiburg/Templates/header}} | ||
+ | <html> | ||
+ | <div id="notebook-page-header"> | ||
+ | <div id="notebook-back" width="100px" > | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/10_September">Previous entry</a> | ||
+ | </div> | ||
+ | <div id="notebook-title"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook"> 11 September </a> | ||
+ | </div> | ||
+ | <div id="notebook-next"> | ||
+ | <a href="https://2011.igem.org/Team:Freiburg/Notebook/12_September">Next entry</a> | ||
+ | </div> | ||
+ | </div> | ||
+ | </html> | ||
+ | |||
+ | |||
+ | ==Commons== | ||
+ | |||
+ | ===Testdigest=== | ||
+ | |||
+ | '''Sandra''' | ||
+ | |||
+ | Testdigest of PR+RFP and PR+GFP | ||
+ | |||
+ | A lot of samples were positive. | ||
+ | |||
+ | PR1-6+RFP: | ||
+ | |||
+ | *11D (PR1+RFP) | ||
+ | *12B (PR2+RFP) | ||
+ | *12C '' | ||
+ | *22B | ||
+ | *31A | ||
+ | *31B | ||
+ | *32D | ||
+ | *51A | ||
+ | *61A | ||
+ | *61D | ||
+ | *62A | ||
+ | *62D | ||
+ | |||
+ | |||
+ | PR1-6+GFP: | ||
+ | |||
+ | *PR2GFP | ||
+ | *PR3GFP | ||
+ | *PR4GFP A | ||
+ | *PR5GFP A/B | ||
+ | *PR6GFP A | ||
+ | |||
==<span style="color:green;">green light receptor</span>== | ==<span style="color:green;">green light receptor</span>== | ||
Line 11: | Line 60: | ||
==<span style="color:blue;">blue light receptor</span>== | ==<span style="color:blue;">blue light receptor</span>== | ||
- | === | + | ===Picking clones=== |
- | '''Investigators: | + | '''Investigators: Sandra''' |
+ | ===Miniprep + Gel=== | ||
+ | '''Investigators: Sandra''' | ||
+ | |||
+ | Stored in blue light box (6 samples). | ||
+ | |||
+ | [[File:Gibson♥-NOT_Freiburg.jpg]] | ||
+ | |||
+ | expected lenght of Gibson-assembly product: 3902bp -> all Gibson assemblies were succesfull exept for G♥-d :) | ||
==<span style="color:red;">red light receptor</span>== | ==<span style="color:red;">red light receptor</span>== | ||
Line 29: | Line 86: | ||
==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
- | === | + | ===M48 in C3 vector=== |
- | '''Investigators: | + | '''Investigators:Theo''' |
+ | |||
+ | |||
+ | Part M48 in C3 Vector was inoculated (5 colonies picked from plate) | ||
+ | |||
+ | <br> | ||
+ | ===Transformation of all possible Lysis composites in C3 vectors=== | ||
+ | High throughput trafos just to be sure that if the sequencing works, I can send the parts in C3 vectors to the registry in time | ||
==<span style="color:grey;">Precipitator</span>== | ==<span style="color:grey;">Precipitator</span>== |
Latest revision as of 00:16, 22 September 2011