Team:Lyon-INSA-ENS/Realisation/Week2

From 2011.igem.org

(Difference between revisions)
 
(25 intermediate revisions not shown)
Line 3: Line 3:
{{INSA-Lyon/styletestaurelie}}
{{INSA-Lyon/styletestaurelie}}
{{Lyon-INSA-ENS/menuhorizontal}}
{{Lyon-INSA-ENS/menuhorizontal}}
-
{{Lyon-INSA-ENS/menuNotebook}}
+
<!--{{Lyon-INSA-ENS/menuNotebookW2}}-->
 +
{{Lyon-INSA-ENS/menuNotebookVertical|Week 2 = actif}}
<html>
<html>
 +
<div style="float : left; margin-top : -10px; margin-left : -200px">
 +
  <a href="https://2011.igem.org/Main_Page" >
 +
      <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
 +
  </a>
 +
</div>
-
<style type="text/css">
+
<body>
-
    .cadre{
+
-
    text-align:center;
+
-
    width : 600px;
+
-
    height:float;
+
-
    margin-left : 50px ;
+
-
    margin-top : 20px;
+
-
    border: 4px solid green;
+
-
    -moz-border-radius: 10px;
+
-
    -webkit-border-radius: 10px;
+
-
    -goog-ms-border-radius: 10px;
+
-
    border-radius: 10px;
+
-
    -moz-box-shadow: rgba(0, 0, 0, 0.3) 0px 0px 12px 7px;
+
-
    -webkit-box-shadow: rgba(0, 0, 0, 0.3) 0px 0px 12px 7px;
+
-
    -goog-ms-box-shadow: rgba(0, 0, 0, 0.3) 0px 0px 12px 7px;
+
-
    box-shadow: rgba(0, 0, 0, 0.3) 0px 0px 12px 7px;
+
-
    }
+
-
</style>
+
    <div id="language";>
 +
        <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="/Team:Lyon-INSA-ENS/Realisation/Week2Fr">Version Fran&ccedil;aise</a>
 +
    </div>
 +
<div class="contenugrand2";>
-
<body>
+
    <br/> <br/>
 +
    <br/>  
-
<div class="contenugrand2";>
 
-
<div class="cadre" ; style="background-color:green;" >
+
<div class="cadre" ; style="background-color:green;margin-left : 8%" >
     <br/>
     <br/>
     <h1 style="color: white;"> Week 2 </h1>     
     <h1 style="color: white;"> Week 2 </h1>     
Line 40: Line 33:
<br/>
<br/>
-
     <p style="text-align : center"> <small> From Monday the 13th of June to Friday the 17th of June 2011 </small> </p>
+
     <p style="text-align : center"> <small> From Monday the 20th of June to Friday the 24th of June 2011 </small> </p>
Line 46: Line 39:
     <br/> <br/>
     <br/> <br/>
-
   <h6 style="text-align :left"> Monday </h6>
+
   <h6 style="text-align :left"> Monday </h6> <HR>
 +
  <br/>
 +
  <p style=" line-height : 1.5em">
 +
<FONT COLOR="orange"> <b> Extraction of NiCoT </b></FONT> <br><br>
 +
 +
One transformed colony (NicoT in NM522) seemed correct, the others were probably satellite colonies without the plasmid.<br/>
 +
Start of 5mL liquid culture of the main colony. <br/>
 +
Plating of the satellite colonies to check the presence of the plasmid <br/>
 +
    </p>
     <br/> <br/>
     <br/> <br/>
 +
    <br/> <br/>
 +
 +
  <h6 style="text-align :left"> Tuesday </h6> <HR>
 +
  <br/>
 +
  <p style=" line-height : 1.5em">
-
  <h6 style="text-align :left"> Tuesday </h6>
+
<FONT COLOR="orange"> <b> Extraction of NiCoT </b></FONT> <br><br>
 +
4 more new clones have grown : start of 5mL liquid cultures on these clones.
 +
    </p>
 +
    <br/> <br/>
     <br/> <br/>     
     <br/> <br/>     
-
  <h6 style="text-align :left"> Wednesday </h6>
+
  <h6 style="text-align :left"> Wednesday </h6> <HR>
 +
  <br/>
 +
  <p style=" line-height : 1.5em">
-
    <br/> <br/>
+
<FONT COLOR="orange"> <b> Extraction of NiCoT </b></FONT> <br><br>
-
 
+
-
<h6 style="text-align :left"> Thursday </h6>
+
 +
Miniprep on the four clones. Verification of plasmid by <b>digestion</b> (EcoRI, HindIII)
 +
    </p>
 +
    <br/> <br/>
     <br/> <br/>
     <br/> <br/>
-
  <h6 style="text-align :left"> Friday </h6>
+
  <h6 style="text-align :left"> Thursday </h6> <HR>
 +
  <br/>
 +
  <p style=" line-height : 1.5em">
-
    <br/> <br/>
+
<FONT COLOR="green"> <b>PCR and mutagenesis of rcn, csgBA, csgEFG</b> </FONT> <br><br>
 +
<b>PCR</b> from genomic DNA from E.coli strain MC4100 to amplify three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)
 +
    </p>   
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <p>
 +
              <a  href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week2"/><font color="grey"><b>Previous Week</b></font></a>
 +
              <a  style = "float : right"; href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week3"/><font color="grey"><b>Next Week</b></font></a>
 +
              <br/>
 +
    </p>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
 +
    <br/> <br/>
</div>
</div>
Line 71: Line 106:
</body>
</body>
</html>
</html>
 +
 +
{{Lyon-INSA-ENS/footer}}

Latest revision as of 23:34, 21 September 2011







Week 2


From Monday the 20th of June to Friday the 24th of June 2011





Monday

Extraction of NiCoT

One transformed colony (NicoT in NM522) seemed correct, the others were probably satellite colonies without the plasmid.
Start of 5mL liquid culture of the main colony.
Plating of the satellite colonies to check the presence of the plasmid





Tuesday

Extraction of NiCoT

4 more new clones have grown : start of 5mL liquid cultures on these clones.





Wednesday

Extraction of NiCoT

Miniprep on the four clones. Verification of plasmid by digestion (EcoRI, HindIII)





Thursday

PCR and mutagenesis of rcn, csgBA, csgEFG

PCR from genomic DNA from E.coli strain MC4100 to amplify three genes : RcnR (cobalt receptor), CsgAB (curli operon, part 1) and CsgEFG (curli operon, part 2)













Previous Week Next Week




















ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/Week2"