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Team:UCL London/Research/Supercoilometer/Experiments
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Revision as of 23:28, 21 September 2011
Building a Supercoilometer in the lab
Aim
To prepare a BioBrick from the tyrT promoter cloned from the E. coli genome and then construct and characterise a device which can detect the level of negative supercoiling in a closed circular plasmid.
Parts used
Part Name |
Parts ID |
Parts Description |
Length (bp) |
Plasmid Backbone |
RBS+CFP.LVA+Terminator |
RBS + cyan fluorescent protein with LVA tag + terminator |
917 |
on pSB1A2 plasmid (2079 bp) - ampicillin resistance |
|
n/a |
standard plasmid backbone (chloramphenicol resistance) |
2070 |
n/a |
