Team:DTU-Denmark-2

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<b>Making Molecular Biology Easier</b>
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<br>
<br>
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<b>We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and <i>Aspergilli </i> ready to plug 'n' play. You can find more information about how this standard works <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly">here</a>.<b>
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<b>A Reporter System</b><br><br>
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As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/fungi" ><i>Aspergillus nidulans</i></a> and the <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/Proofofconcept/mammalian"> mammalian cell line U-2 OS </a> with great success.</p>
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<b>Flexibility</b><br><br>
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Standardization entails rigidity. Therefore we have written a <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization" >guide</a> that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.</p>
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<b>Applications</b><br><br>
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<a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/introduction#Application%20area">Here</a> you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.</p>
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<b>Data Page</b><br><br>
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<b> Why Plug 'n' Play with DNA?</b><br>
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<img src="https://static.igem.org/mediawiki/2011/c/c3/AspergillusDTU_billed.jpg" width="187px" height="50px"> </img><br>
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We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans.  
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You can find an overview of our project on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/data_page">Data Page</a>. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.</p>
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<b>Achievements</b><br><br>
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Designing a novel assembly standard and creating a reporter system for <i>Aspergillus nidulans</i> and mammalian cells are our main achievements. You can read more about our accomplishments on <a href="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievements"> this page</a>.</p>
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<b>The Team</b><br><br>
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<img src="https://static.igem.org/mediawiki/2011/thumb/f/fd/DSC_0349.jpg/800px-DSC_0349.jpg" width="187px" height="50px"> </img><br>
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We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our <a href="https://2011.igem.org/Team:DTU-Denmark-2/Team">Team Page</a>.</p>
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<a href="https://igem.org/Team_Wikis?year=2011"><b>iGEM 2011 Wiki Main Page</b></a>
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   slogan[9] = "Plug’n’play with DNA will change the world today";
   slogan[9] = "Plug’n’play with DNA will change the world today";
   slogan[10] = "Plug’n’play can hold your favorite DNA";
   slogan[10] = "Plug’n’play can hold your favorite DNA";
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   slogan[11] = "Mix it, Heat it, Use it, it's that easy";
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   slogan[11] = "Mix it, heat it, use it, it's that easy";
   slogan[12] = "Avoid restriction, use the plug 'n' play edition";
   slogan[12] = "Avoid restriction, use the plug 'n' play edition";
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   slogan[13] = "Make a smart desision, use the plug 'n' play edition";
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   slogan[13] = "Make a smart decision, use the plug 'n' play edition";
   slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
   slogan[14] = "Plug’n’play with DNA is complete, it is easy, cool and smart, indeed";
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<li> We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi. </li>
 
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<li> We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence. </li>
 
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<li> We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy. </li>
 
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<li>We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/submitted biobricks> List </a>.
 
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<li>We applied for a gold medal by fulfilling the <a hret="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievement"> requirements listed</a> by iGEM 2011. </li>
 
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<b> Sponsored by</b><br><br>
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<img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
<img src="https://static.igem.org/mediawiki/2011/0/0c/Alk_Logo.jpg" with="80px"height="80px"> </img> &nbsp;
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Latest revision as of 23:10, 21 September 2011


Making Molecular Biology Easier


We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can be gathered without the use of restriction enzymes and ligase. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergilli ready to plug 'n' play. You can find more information about how this standard works here.


A Reporter System

As proof of concept we have created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We expressed and localized fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with great success.
Flexibility

Standardization entails rigidity. Therefore we have written a guide that allows the researcher to customize the system, so proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc. The only limitation to this is your creativity.
Applications

Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the research areas, where this system is especially advantageous.


Data Page

You can find an overview of our project on our Data Page. Here you also find our favorite submitted biobricks, and the biobricks we characterized during the summer.
Achievements

Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page.
The Team

We are five girls that have been working on this project for three months with support from our three supervisors. You can learn more about us, and how we started an iGEM team on our Team Page.

iGEM 2011 Wiki Main Page









Sponsored by

  

 

   

       

Comments or questions to the team? Please

Retrieved from "http://2011.igem.org/Team:DTU-Denmark-2"