Team:Bilkent UNAM Turkey/Collaboration

From 2011.igem.org

(Difference between revisions)
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<p>FATIH_TURKEY<p>
<p>FATIH_TURKEY<p>
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We have assisted the FATIH_TURKEY ([https://2011.igem.org/Team:Fatih_Turkey]) team by providing them with both
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We have assisted the FATIH_TURKEY (link) team by providing them with both
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unmodified ''B.subtilis'' and competent ''B.subtilis'' cultures, which they
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unmodified B.subtilis and competent B.subtilis cultures, which they
required in order for their project to proceed. We also donated materials
required in order for their project to proceed. We also donated materials
such as LB, antibiotic and agar to this team so that they may conduct
such as LB, antibiotic and agar to this team so that they may conduct
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(even though they were diligent in purchasing the needed chemicals
(even though they were diligent in purchasing the needed chemicals
immediately, orders may take up to 4 months to be delivered in Turkey, a
immediately, orders may take up to 4 months to be delivered in Turkey, a
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fact we find most unfortunate.)'' B.subtilis'' cultures were kindly donated to
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fact we find most unfortunate.) B.subtilis cultures were kindly donated to
the iGEM team from one of lab members (Alper Ozkan) and delivered by hand
the iGEM team from one of lab members (Alper Ozkan) and delivered by hand
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to a Fatih iGEM member.
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to a Fatih iGEM member.<p>
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We must also thank them for providing us with biobrick vectors for ''B.
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We must also thank them for providing us with biobrick vectors for B.
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subtilis''. The vectors we have obtained from their team are BBa_K541026
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subtilis. The vectors we have obtained from their team are BBa_K541026
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(Reflectin 1A (SacB) and LALF (LipA) protein for ''B.subtilis''), BBa_K090401
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(Reflectin 1A (SacB) and LALF (LipA) protein for B.subtilis), BBa_K090401
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(Gram-positive Shuttle Vector for Episomal Expression), BBa_K090402 (''B.
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(Gram-positive Shuttle Vector for Episomal Expression), BBa_K090402 (B.
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subtilis'' Episomal Vector with Constitutive GFP) and BBa_K090403
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subtilis Episomal Vector with Constitutive GFP) and BBa_K090403
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(Gram-positive Shuttle Vector for Chromosomal Integration)
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(Gram-positive Shuttle Vector for Chromosomal Integration)<p>
We planned to use these vectors for a project concerning the expression of
We planned to use these vectors for a project concerning the expression of
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mussel adhesion foot proteins ''B. subtilis'', but did not get the chance to
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mussel adhesion foot proteins B. subtilis, but did not get the chance to
utilize them because our relatively small team scarcely had the time to
utilize them because our relatively small team scarcely had the time to
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work with both ''C. reinhardtii'' and ''B. subtilis''. We have opted to continue
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work with both C. reinhardtii and B. subtilis. We have opted to continue
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with ''C. reinhardtii'', of which preliminary tests yielded promising results.
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with C. reinhardtii, of which preliminary tests yielded promising results.
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<p>
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+
-
 
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Revision as of 22:50, 21 September 2011

FATIH_TURKEY

We have assisted the FATIH_TURKEY (link) team by providing them with both unmodified B.subtilis and competent B.subtilis cultures, which they required in order for their project to proceed. We also donated materials such as LB, antibiotic and agar to this team so that they may conduct their preliminary experiments before they obtained the necessary reagents (even though they were diligent in purchasing the needed chemicals immediately, orders may take up to 4 months to be delivered in Turkey, a fact we find most unfortunate.) B.subtilis cultures were kindly donated to the iGEM team from one of lab members (Alper Ozkan) and delivered by hand to a Fatih iGEM member.

We must also thank them for providing us with biobrick vectors for B. subtilis. The vectors we have obtained from their team are BBa_K541026 (Reflectin 1A (SacB) and LALF (LipA) protein for B.subtilis), BBa_K090401 (Gram-positive Shuttle Vector for Episomal Expression), BBa_K090402 (B. subtilis Episomal Vector with Constitutive GFP) and BBa_K090403 (Gram-positive Shuttle Vector for Chromosomal Integration)

We planned to use these vectors for a project concerning the expression of mussel adhesion foot proteins B. subtilis, but did not get the chance to utilize them because our relatively small team scarcely had the time to work with both C. reinhardtii and B. subtilis. We have opted to continue with C. reinhardtii, of which preliminary tests yielded promising results.

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