Team:Imperial College London/Templates/Kill

From 2011.igem.org

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<html>
<html>
<head>
<head>
 +
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
   <meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
   <script type="text/javascript">
   <script type="text/javascript">
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     var last = path.split("/");
     var last = path.split("/");
     if ( last[1] ) {
     if ( last[1] ) {
-
       $('.underlinemenu ul li a[href*="' + last[1] + '"]').addClass("curlink").css("border-bottom","3px solid #225323");
+
       $('.underlinemenu ul li a[href*="' + last[1] + '"]').addClass("curlink").css("background-color","#648765");
     }
     }
     else {
     else {
-
       $('.underlinemenu ul li a[href$="' + path + '"]').addClass("curlink").css("border-bottom","3px solid #225323");
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       $('.underlinemenu ul li a[href$="' + path + '"]').addClass("curlink").css("background-color","","#648765");
     }
     }
     });
     });
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.underlinemenu ul li a{
.underlinemenu ul li a{
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color: #225323;
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color: #ffffff;
 +
background-color: #225323;
padding: 6px 3px 4px 3px; /*top padding is 6px, bottom padding is 4px*/
padding: 6px 3px 4px 3px; /*top padding is 6px, bottom padding is 4px*/
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margin-right: 20px; /*spacing between each menu link*/
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margin-right: -4px; /*spacing between each menu link*/
text-decoration: none;
text-decoration: none;
}
}
.underlinemenu ul li a:hover, .underlinemenu ul li a.selected{
.underlinemenu ul li a:hover, .underlinemenu ul li a.selected{
-
border-bottom: 3px solid #225323; /*bottom border is 3px*/
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background-color: #648765;
}
}
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<body>
<body>
<hr style="color:#BDCBBD; height:3px;" />
<hr style="color:#BDCBBD; height:3px;" />
 +
<img class="border" src="https://static.igem.org/mediawiki/2011/5/5d/New_storyboard_v2-09.png" width="170px" style="float:right;margin:23px 20px 0px 20px;" />
<h1>Module 3: Gene Guard</h1>
<h1>Module 3: Gene Guard</h1>
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<p><b>Containment is a serious issue concerning the release of GMOs into the environment. To prevent horizontal gene transfer of the genes we have expressed in our chassis, we have developed a toxin/anti-toxin system based on the genes encoding holin and anti-holin. We are designing our bacteria to contain anti-holin (the anti-toxin) within its genome and holin & endolysin on the plasmid with the Phyto-Route and Auxin Xpress genes. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin would be transferred without anti-holin, effectively killing the recipient cell.</b></p>
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<p><b>Containment is a serious issue concerning the release of genetically modified organisms (GMOs) into the environment. To prevent horizontal gene transfer of the genes we are expressing in our chassis, we have developed a system based on the genes encoding holin, anti-holin and endolysin. We are engineering anti-holin into the genome of our chassis, where it acts as an anti-toxin, and holin and endolysin on plasmid DNA. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin will be transferred without anti-holin, rendering the recipient cell non-viable and effectively containing the Auxin Xpress and Phyto-Route genes in our chassis.</b></p>
<br/>
<br/>
<div class="underlinemenu">
<div class="underlinemenu">
Line 63: Line 66:
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Modelling">Modelling</a></li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Modelling">Modelling</a></li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Assembly">Assembly</a></li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Assembly">Assembly</a></li>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Testing">Testing</a></li>
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<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Testing">Testing & Results</a></li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Future">Future Work</a></li>
<li><a href="https://2011.igem.org/Team:Imperial_College_London/Project_Gene_Future">Future Work</a></li>
</ul>
</ul>

Latest revision as of 22:42, 21 September 2011


Module 3: Gene Guard

Containment is a serious issue concerning the release of genetically modified organisms (GMOs) into the environment. To prevent horizontal gene transfer of the genes we are expressing in our chassis, we have developed a system based on the genes encoding holin, anti-holin and endolysin. We are engineering anti-holin into the genome of our chassis, where it acts as an anti-toxin, and holin and endolysin on plasmid DNA. In the event of horizontal gene transfer with a soil bacterium, holin and endolysin will be transferred without anti-holin, rendering the recipient cell non-viable and effectively containing the Auxin Xpress and Phyto-Route genes in our chassis.