Team:Groningen/project characterisation mu lasr

From 2011.igem.org

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Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.  
Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.  
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[[File:lasr2.png]]
 
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==Leakiness of the LasR autoinducing loops==
==Leakiness of the LasR autoinducing loops==
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[[File:lasR1.png]]
 
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[[File:lasR2.png]]
 
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[[File:lasR3.png]]
 
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[[File:lasR4.png]]
 
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[[File:lasR5.png]]
 
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[[File:lasR6.png]]
 
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[[File:lasR7.png]]
 
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[[File:lasR8.png]]
 
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[[File:lasR9.png]]
 
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[[File:lasR10.png]]
 
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[[File:lasR11.png]]
 
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Revision as of 22:34, 21 September 2011


LasR Autoinducing Loops

Our second memory unit consists of a LasR gene(Bba_C0179) driven the LasB promoter(Bba_R0079). LasR only activates transcription in the presence of HSL, this was added to the medium in a concentration of 100nM.

Leakiness of the LasR autoinducing loops

no-tag DAS LVA
RBS 0,07

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607031 BBa_K607031]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607027 BBa_K607027]

LasR1.png

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607023 BBa_K607023]

Legenda.png
RBS 0,3

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607032 BBa_K607032]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607028 BBa_K607028]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607024 BBa_K607024]

RBS 0,6

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607033 BBa_K607033]

LasR7.png

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607029 BBa_K607029]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607025 BBa_K607025]

RBS 1

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607034 BBa_K607034]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607030 BBa_K607030]

[http://partsregistry.org/wiki/index.php?title=Part:BBa_K607026 BBa_K607026]

Figure 4. Flow cytometer data taken at different time-points during exponential growth of cells carrying different cI autoinducing loops.




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