Team:Bilkent UNAM Turkey

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<p> Kardesim su yazıları degistirin artik. <p>
<p> Kardesim su yazıları degistirin artik. <p>
          
          
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<p><span lang=3DTR>Yenisini gonderdik ekleyin We aim for the genetic modification of the unicellular microalga <i
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Our aim is to modify the unicellular microalga <i>Chlamydomonas reinhardtii</i> for effective trinitrotoluene (TNT)<br> biodegradation by expression of Enterobacter cloacae nitroreductase gene nfsI.  <i>C. reinhardtii</i> is a ubiquitous <br>species capable of thriving in soil, freshwater and marine environments, making this alga a suitable choice for<br> removal of TNT from a wide variety of biomes. We have opted for the use of nfsI as the gene in question is<br> a well-characterized nitroreductase with a known sequence. Our experimental procedure involves (a) development<br> of a synthetic nfsI gene with flanking prefix and suffixes of standard Biobricks, (b) ligation of this sequence<br> to the C. heinhardtii expression vector pRbcBRL, (c) transfection of <i>C. reinhardtii</i> with the recombinant plasmid<br> and (d) TNT tolerance and biodegradation capacity assessment of the modified alga. <br>
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style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> by introducing the
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<i style=3D'mso-bidi-font-style:normal'>nfsI</i> gene belonging to the bacterium <i
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style=3D'mso-bidi-font-style:normal'>Enterobacter cloacae</i> in order to
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investigate how nitroreductase expressing-microalgae respond to trinitrotoluene
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(TNT) exposure. Our experimental design is as follows:
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firstly, obtain a synthetic gene of <i style=3D'mso-bidi-font-style:normal'>nfsI</i> with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector with
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appropriate expression and selection system for <i style=3D'mso-bidi-font-style:
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normal'>Chlamydomonas reinhardtii</i> and obtain pRbcnfsI. Then, <i style=3D'mso-bidi-font-style:normal'>Chlamydomonas reinhardtii</i> will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on
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biological degradation of TNT will be investigated.<b style=3D'mso-bidi-font-weight:normal'><o:p
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Revision as of 22:14, 21 September 2011

 
PROJECT
Overview
TEAM
Gallery Team_Page
LABWORKS
Protocols Experiment Lab Safety
HUMAN PRACTICE
BBQ Party Team Meetup iGEM Everywhere Collaboration
BIOBRICK PARTS ATTRIBUTIONS

    Biodegradation of TNT and TNT derivatives by nfsI-transfected Chlamydomonas Reinhardtii

    Abstract

    Kardesim su yazıları degistirin artik.

    Our aim is to modify the unicellular microalga Chlamydomonas reinhardtii for effective trinitrotoluene (TNT)
    biodegradation by expression of Enterobacter cloacae nitroreductase gene nfsI. C. reinhardtii is a ubiquitous
    species capable of thriving in soil, freshwater and marine environments, making this alga a suitable choice for
    removal of TNT from a wide variety of biomes. We have opted for the use of nfsI as the gene in question is
    a well-characterized nitroreductase with a known sequence. Our experimental procedure involves (a) development
    of a synthetic nfsI gene with flanking prefix and suffixes of standard Biobricks, (b) ligation of this sequence
    to the C. heinhardtii expression vector pRbcBRL, (c) transfection of C. reinhardtii with the recombinant plasmid
    and (d) TNT tolerance and biodegradation capacity assessment of the modified alga.

  • Something about spirit of being team

  • In this summer; we learned about iGEM, how it works, what the biggest part of competition is and how we can win a medallion.

    When we were newbie, we organized a lot of meeting to find out our main project than we got idea for improving human welfare so we choose an idea from brainstorm results. We searched to get a gene to work on. As a result; lab works began. We added our protocols which are helpful to transform Chlamydomonas reinhardtii and Escherichia coli. Basic cloning protocols are available on our protocol page. Experiments and safety questions also available from link at the top section.

  • IGEM Project is our number one priority. As we worked really hard for our project it is important for us to make our dreams come true which is taking a gold medal. Questioning different items like experiment time, materials and methods and the efficiency of the experiments improved our skills and we started to look as a scientist to problems we faced. There were lots of challenging parts and sometimes we thought to quit but we listened to “Eye of The Tiger” and we did not give up from our experiments we delayed them yes, but we never gave up.

    IGEM team- meet-up was a great opportunity for us to communicate with the other groups, understood their projects and share our ideas. As we had a chance for an interview with Anatolia Agency, we also used media power to get attention to our project, iGEM and the importance of synthetic biology.

    Apart from that we did not forget having fun apart from our lab. As we planned a BBQ party, it was also a great chance for us to relax and gain some energy for our experiments, I did not even mention about the delicious foods and how joyful was our activities. Also like in iGEM team meeting up, we had a chance to introduce our project and main aim to other scientists and researchers in different areas.

    Links at above lead you to specific event and its photos suppose to be at that link. If not, look at gallery link. Thanks and get great time.

    Favorite Parts

    BBa_K596001

    BBa_K596004

    DNA Submission to Registry

    bunu koyun yanlis olmus https://2011.igem.org/File:DNA_submission_to_registry.JPG

  • We are really grateful to Gulce Itir Percin, Aydan Torun,Ömer Faruk Sarıoglu and Zeynep E. Ulger for their help and especially guidance of Mrs. Ulger about unknown lab equipments. Prof. Dr. Tayfun Özçelik and Assistant Prof. Ali Güre are helped us to start iGEM team. Assistant Prof. Ayşe Tekinay and her lab were assisted our project a lot. Also we thanks to other members of SBL and NBT for patience.

    Sponsors

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