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Team:TU-Delft/Project/Production
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The basis of our project is the production of Mfp-5 itself. For this, we use a BioBrick made by the iGEM team of Berkeley Wetlab 2009 (BBa_K197034). They attached Mfp-5 to various membrane proteins with varying linkers in order to research the influence of these linkers on the activity and to find the best combination for optimization. For our project, we chose the combination with the highest activity: Mfp-5 linked to OmpA (Outer membrane protein A) with the linker GFP-LVA | The basis of our project is the production of Mfp-5 itself. For this, we use a BioBrick made by the iGEM team of Berkeley Wetlab 2009 (BBa_K197034). They attached Mfp-5 to various membrane proteins with varying linkers in order to research the influence of these linkers on the activity and to find the best combination for optimization. For our project, we chose the combination with the highest activity: Mfp-5 linked to OmpA (Outer membrane protein A) with the linker GFP-LVA | ||
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Latest revision as of 20:18, 21 September 2011
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Home
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The Project
What we are doing
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The Team
Who we are
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Notebooks
What we did
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Human Practice
Awareness
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Safety
Responsibilty
Production
The basis of our project is the production of Mfp-5 itself. For this, we use a BioBrick made by the iGEM team of Berkeley Wetlab 2009 (BBa_K197034). They attached Mfp-5 to various membrane proteins with varying linkers in order to research the influence of these linkers on the activity and to find the best combination for optimization. For our project, we chose the combination with the highest activity: Mfp-5 linked to OmpA (Outer membrane protein A) with the linker GFP-LVA
