"
Team:Bilkent UNAM Turkey/Experiment
From 2011.igem.org
(Difference between revisions)
| Line 2: | Line 2: | ||
We ordered our genes. We optimized codon usage because of gene taken from <i>E. cloacae</i>. | We ordered our genes. We optimized codon usage because of gene taken from <i>E. cloacae</i>. | ||
<a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Codon_Optimization"><p>Codon Usage </a> | <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Codon_Optimization"><p>Codon Usage </a> | ||
| - | Then we did <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Digestion_%28Gel_Electrophoresis%29">restriction digestion and gel | + | Then we did <a href="https://2011.igem.org/Team:Bilkent_UNAM_Turkey/Digestion_%28Gel_Electrophoresis%29">restriction digestion and gel electrophoresis</a>.<br> |
We repeat this step a lot because of failuire.I put right result<br> | We repeat this step a lot because of failuire.I put right result<br> | ||
<img src="https://static.igem.org/mediawiki/2011/3/3e/Restriction_products_of_minipreps_of_pRbcnfsI_unam.jpg" align="center"></img> | <img src="https://static.igem.org/mediawiki/2011/3/3e/Restriction_products_of_minipreps_of_pRbcnfsI_unam.jpg" align="center"></img> | ||
| + | We used standard <a href="http://tools.invitrogen.com/content/sfs/manuals/purelink_pro96_plasmid_kit_man.pdf">gel purification kit</a> | ||
</html> | </html> | ||
Revision as of 20:12, 21 September 2011
We ordered our genes. We optimized codon usage because of gene taken from E. cloacae.
Codon Usage
We repeat this step a lot because of failuire.I put right result
We used standard gel purification kit
