Team:KULeuven/Data
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<h2>We've also characterized the following parts</h2> | <h2>We've also characterized the following parts</h2> | ||
- | <li> 1. <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584004" target="blank"> <b>BBa_K584004</b> </a> - <b> HybB promoter + GFP generator</b> (K.U.Leuven, iGEM 2011): we created this biobrick | + | <li> 1. <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584004" target="blank"> <b>BBa_K584004</b> </a> - <b> HybB promoter + GFP generator</b> (K.U.Leuven, iGEM 2011): we created this biobrick and tested it..<br><br> |
<li> 2. <a href="http://partsregistry.org/Part:BBa_K091107:Experience" target="blank"> <b>BBa_K091107</b> </a> - <b> pLux/cI Hybrid Promoter</b> (K.U.Leuven, iGEM 2011): We wanted to use this promoter at the inception of our project, however on a 1% agarose gel we saw one fragment of a different height than reported by previous iGEM competitions.<br><br> | <li> 2. <a href="http://partsregistry.org/Part:BBa_K091107:Experience" target="blank"> <b>BBa_K091107</b> </a> - <b> pLux/cI Hybrid Promoter</b> (K.U.Leuven, iGEM 2011): We wanted to use this promoter at the inception of our project, however on a 1% agarose gel we saw one fragment of a different height than reported by previous iGEM competitions.<br><br> | ||
<li> 3. <a href="http://partsregistry.org/Part:BBa_K091104:Experience" target="blank"> <b>BBa_K091104</b> </a> - <b> pLac/Mnt Hybrid Promoter</b> (K.U.Leuven, iGEM 2011): We wanted to use this promoter at the inception of our project, however on a 1% agarose gel we saw one fragment at a different height than reported by previous iGEM competitions. | <li> 3. <a href="http://partsregistry.org/Part:BBa_K091104:Experience" target="blank"> <b>BBa_K091104</b> </a> - <b> pLac/Mnt Hybrid Promoter</b> (K.U.Leuven, iGEM 2011): We wanted to use this promoter at the inception of our project, however on a 1% agarose gel we saw one fragment at a different height than reported by previous iGEM competitions. |
Revision as of 20:06, 21 September 2011
![](http://homes.esat.kuleuven.be/~igemwiki/images/bg_team02.jpg)
Data page
How does E.D. Frosti work?
![](http://homes.esat.kuleuven.be/~igemwiki/images/data/figure01.png)
The first illustration is a global overview of our project in which you can see how the plasmids (and the parts) function in our system. The next illustration shows the plasmids in more detail, with the favorite new parts and the characterized pre-existing parts.
![](http://homes.esat.kuleuven.be/~igemwiki/images/data/figure02.png)
Plasmid 1 contains the bricks necessary to induce expression of the red color (via the CrtEBI system) and ice-nucleating protein (INP) in a lactose-dependent manner. For this plasmid, the INP has been cloned (BBa_K584024) and tested for functionality by putting it behind the constitutive (BBa_K584027) and lactose-inducible ( BBa_K584028 ) promoter.
Plasmid 2 contains the bricks that induce the expression of the black color (via the melA system) and antifreeze protein (AFP) in a arabinose-dependent manner. For this plasmid, the AFP has been cloned ( BBa_K584020) and tested for functionality by putting it behind the constitutive promoter (BBa_K584026).
Plasmid 3 contains the necessary bricks for our cell death mechanism. Briefly, lactose or arabinose will generate a ribolock-ceaB construct ( BBa_K584015 and BBa_K584016).The Ribokey can be activated by a cold shock( BBa_K584014). For this plasmid we cloned the lactose- and arabinose-inducible ceaB construct ( BBa_K584016 and BBa_K584015 resp.) and characterized the cold shock promoter using the Biobrick BBa_K584004.
![](http://homes.esat.kuleuven.be/~igemwiki/images/data/figure03.png)