Team:Wageningen UR/Safety/Three

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== Safety ==
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=== Accidental release to the environment ===
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There are several scenarios in which unintentional release of genetically modified material could take place.
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Labeling of the lab equipment and glassware used is necessary to prevent loss and improper waste disposal by a fellow researcher. The following problems are not as easy to prevent.
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The air filtering system will have a hard time in keeping aerosols in the lab when a window gets broken. If this happens through a thunderstorm, an electricity break-down is not unlikely and would increase the chance of release furthermore. Under Good Microbial Practice, though, the formation of aerosols is prevented as much as possible, thus the combination of these probabilities leaves a total probability that is not that high.
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== Biosafety and biosecurity considerations ==
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In transport from one end of  the lab to the other, there is however a chance the transporter falls and cuts themself with the broken glassware containing hazardous biological material. Infection is a high probability in this case. For our project prophylaxes would be available, which reduce the chance on the development of a disease.
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=== Would the materials used in your project and/or your final product pose risks to the safety and health of the general public if released by design or accident? ===
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It should furthermore be reported to the Minister of ‘Housing Spatial Planning and the Environment’ and, involved, institutions and direct actions should be taken if it is found that an autoclave has been malfunctioning. The chance that this happens however is as large to happen in our project as it would be for other projects. Still it has to be checked as frequently as possible if the autoclave is functioning.
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Using the ''E. coli'' Top 10 strain as the cell chassis would prevent the BioBrick system from being expressed in the environment. The strain is not expected to grow under the harsh conditions outside the laboratory. The BioBrick part DNA, though, might be taken up by other bacteria via natural genetic transformation. As was found in the section [https://2011.igem.org/Team:Wageningen_UR/Safety/One in the lab], so far no pathogenic species are found in the soil that used the BioBrick part’s quorum sensing molecule. Since the BioBrick system is not likely to reach marine environments, where potentially interacting pathogens occur, and does not produce toxic compounds, we assume that the BioBrick system in this cell chassis is quite safe and the risk to the health of the general public is low.
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The usage of antibiotic resistance markers, to select for tetracycline resistance for example, increases the chance of spreading antibiotic resistance to pathogens. Conjugation and transduction is a way of  DNA transfer between bacteria. There is a chance the antibiotic resistance genes end up in a pathogenic bacterium that isn’t intrinsically resistant to antibiotics. However, this hazard is present in most molecular microbiology research.
 
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Some hazards have already been mentioned by giving the probabilities of them, but in the following paragraph a closer look to harmful effects will be taken.
 
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Latest revision as of 19:43, 21 September 2011

Building a Synchronized Oscillatory System

Biosafety and biosecurity considerations

Would the materials used in your project and/or your final product pose risks to the safety and health of the general public if released by design or accident?

Using the E. coli Top 10 strain as the cell chassis would prevent the BioBrick system from being expressed in the environment. The strain is not expected to grow under the harsh conditions outside the laboratory. The BioBrick part DNA, though, might be taken up by other bacteria via natural genetic transformation. As was found in the section in the lab, so far no pathogenic species are found in the soil that used the BioBrick part’s quorum sensing molecule. Since the BioBrick system is not likely to reach marine environments, where potentially interacting pathogens occur, and does not produce toxic compounds, we assume that the BioBrick system in this cell chassis is quite safe and the risk to the health of the general public is low.

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