From 2011.igem.org
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| - | == Gel preparation (1% gel) (100 ml of the buffer) ==
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| - | <ol>
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| - | <li value="a">100 ml of 1x TBE buffer.</li>
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| - | <li value="b">10 μl of ethidium bomide (10 mg/ml).</li>
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| - | <li value="c">1 g of agarose.</li>
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| - | <li value="d">Assembled gel container.</li>
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| - | </ol>
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| - | # Mix buffer with agarose and heat in microwave until the solution is clear
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| - | # Add 10μl of ethidium bromide (10mg/ml).
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| - | # Pour solution to the gel container and leave it to solidify (30-45 min).
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| - | # Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer.
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| - | Gel electrophoresis:
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| - | <ol>
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| - | <li value="i">2μl of DNA sample.</li>
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| - | <li value="ii">3 μl of distilled water.</li>
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| - | <li value="iii">1μl of 6x loading dye.</li>
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| - | <li value="iv">4.2 μl of Gene Ruler DNA ladder mix from Fermentas.</li>
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| - | </ol>
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| - | It is recommended to use 7V for each cm of the gel length and to run the gel for 45 min.
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Latest revision as of 19:08, 21 September 2011
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