Team:DTU-Denmark/Gel preparation and gel electrophoresis

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== Gel preparation (1% gel) (100 ml of the buffer) ==
 
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# 100 ml of 1x TBE buffer .
 
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# 10 μl of ethidium bomide (10 mg/ml).
 
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# 1 g of agarose.
 
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# Assembled gel container.
 
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# Mix buffer with agarose and heat in microwave until the solution is clear
 
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# Add 10μl of ethidium bromide (10mg/ml).
 
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# Pour solution to the gel container and leave it to solidify (30-45 min).
 
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# Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer.
 
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Gel electrophoresis:
 
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# 2μl of DNA sample.
 
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# 3 μl of distilled water.
 
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# 1μl of 6x loading dye.
 
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# 4.2 μl of  Gene Ruler DNA ladder mix from Fermentas.
 
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It is recommended to use 7V for each cm of the gel length and to run the gel for 45 min.
 

Latest revision as of 19:08, 21 September 2011

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