From 2011.igem.org
(Difference between revisions)
|
|
| (57 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{:Team:DTU-Denmark/Templates/Standard_page_begin|Methods}}
| |
| - | == Lab ==
| |
| - | \section{Overview}
| |
| - | Our project is a proof of concept project, showing that the \textit{E. coli} sRNA \textit{sroB} and the intergenic sRNA in the \textit{chbBCARG} gene can be used to control gene expression by targeting the \textit{ybfM} Shine-Delgarno, and that these sRNAs can be rationally designed to targeted other Shine-Delgarnos
| |
| | | | |
| - | The experimental part of the project can be broken into 3 distinct parts, which combine to form the complete project:
| |
| - | \begin{itemize}
| |
| - | \item Construction of plasmids
| |
| - | \item Strain construction
| |
| - | \item Improving the \textit{araBAD} promoter
| |
| - | \end{itemize}
| |
| - | \paragraph{Construction of plasmids}
| |
| - | necessary for testing our system involves taking the native system from \textit{E. coli}, as well as a slightly modified system and putting them on plasmids that let us both control the expression of these components and measure the output of the system.
| |
| - | \paragraph{Strain construction}
| |
| - | involves deleting the original genes from the chromosome of a \textit{E. coli} W3110 strain. Since we use the original genes, these need to be deleted from the chromosome to prevent them from interfering with our measurements.
| |
| - | \paragraph{Improving the \textit{araBAD} promoter}
| |
| - | entails expanding the dynamic range of this promoter by modifying the -10 and -35 sequence of the promoter, as well as randomly changing the nucleotide sequence around and in between these sequences. Since the \textit{araBAD} promoter is used in our project improving this promoter could lead to even finer control of our system.
| |
| - |
| |
| - | == Notebook ==
| |
| - | {|
| |
| - | |Orange||Apple||more
| |
| - | |-
| |
| - | |Bread||Pie||more
| |
| - | |-
| |
| - | |Butter||Ice<br />cream||and<br />more
| |
| - | |}
| |
| - |
| |
| - |
| |
| - | {{:Team:DTU-Denmark/Templates/Standard_page_end}}
| |
Latest revision as of 19:04, 21 September 2011
"
