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Team:ULB-Brussels/parts
From 2011.igem.org
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The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase. <br /> | The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase. <br /> | ||
It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid. </p> | It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid. </p> | ||
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<h1><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K551001>Part:BBa_K551001</a>: pINDEL</h1> | <h1><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K551001>Part:BBa_K551001</a>: pINDEL</h1> | ||
<p> </p> | <p> </p> | ||
Revision as of 17:50, 21 September 2011
Parts submitted
Part:BBa_K551001: pINDEL
The pINDEL plasmid is composed of 2 functional units:
- the IN function which is composed of the gam, exo and bet genes coding for the lambda Red recombinase system
- the DEL function which is based on the flp gene encoding the FLP site-specific recombinase and expressed at 42°C
Another feature of pINDEL is that its replication origin is not functional at 42°C due to the thermosensitivity of the RepA101Ts protein. This protein initiates replication at the ori site in permissive conditions (30°C) and is inactive at 42°C.
The deletion function and the thermosensitivity of the plasmid both could be shown to be working as intended (see functional parameters). The insertion function couldn't be properly tested
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