Team:Paris Liliane Bettencourt/Notebook/2011/08/10/

(Difference between revisions)

Latest revision as of 16:27, 21 September 2011

Team IGEM Paris 2011

Gel of PCR 2 -> 9 = LacI:GFP - 12 to 17 = tetR:YFP

Gel of PCR 1 -> 2 = tetR:YFP - 5 -> 12 = LacO Array - 15 -> 17 = tetO Array

Gel of PCR 1 -> 4 = tetO Array

We have products and lines that we expected !

We tried to insert the Biobricks "pVeg+SpoVG T7 RFP T7 term and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3

-K606030

-K606036

-K606030 by EcoR1 and Pst1

-T7 Autoamplifier system + Reporter (K606036) by EcoR1 and Pst1

-pHM3 by EcoR1 and Pst1

-K606030 & pHM3

-K606036 & pHM3

@@ Line 1: / Line 1: @@
+{{:Team:Paris_Bettencourt/tpl_test}}
 == Kevin ==
 === PCR Results ===
-[[File:gelpcrdl1.jpg|450px|thumb|center|Gel of PCR 2 -> 9 = LacI:GFP | 12 to 17 = tetR:YFP]]
+[[File:gelpcrdl1.jpg|450px|thumb|center|Gel of PCR 2 -> 9 = LacI:GFP - 12 to 17 = tetR:YFP]]
-[[File:gelpcrdl2.jpg|450px|thumb|center|Gel of PCR 1 -> 2 = tetR:YFP | 5 -> 12 = LacO Array | 15 -> 17 = tetO Array]]
+[[File:gelpcrdl2.jpg|450px|thumb|center|Gel of PCR 1 -> 2 = tetR:YFP - 5 -> 12 = LacO Array - 15 -> 17 = tetO Array]]
 [[File:gelpcrdl3.jpg|450px|thumb|center|Gel of PCR 1 -> 4 = tetO Array]]
+We have products and lines that we expected !
+==Mathias & Laura==
+We tried to insert the Biobricks "pVeg+SpoVG T7 RFP T7 term and reporter for Bacillus" and "T7 Autoamplifier system + Reporter" into pHM3
+===Miniprep===
+-K606030
+-K606036
+===Digestion and gel extraction===
+-K606030 by EcoR1 and Pst1
+-T7 Autoamplifier system + Reporter (K606036) by EcoR1 and Pst1
+-pHM3 by EcoR1 and Pst1
+===Ligation and transformation===
+-K606030 & pHM3
+-K606036 & pHM3