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Team:Paris Liliane Bettencourt/Notebook/2011/07/12/
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'''Plasmid cultures from David Lane (LacO/tetO array)''' <br> | '''Plasmid cultures from David Lane (LacO/tetO array)''' <br> | ||
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-pDAG464 -> CmR , KanR - 4 - lacO array<br> | -pDAG464 -> CmR , KanR - 4 - lacO array<br> | ||
-pSG20 -> Amp - 5 - gfp:lacI<br> | -pSG20 -> Amp - 5 - gfp:lacI<br> | ||
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| + | ==Mathias & Laura== | ||
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| + | After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo. | ||
Latest revision as of 15:53, 21 September 2011
Kevin
Plasmid cultures from David Lane (LacO/tetO array)
-10mL LB + 10μL Antibiotics overnight
-pDAG480 -> CmR - 1 - tetO array
-pDAG479 -> CmR - 2 - tetO array
-pFX234 -> Amp - 3 - yfp:tetR
-pDAG464 -> CmR , KanR - 4 - lacO array
-pSG20 -> Amp - 5 - gfp:lacI
Mathias & Laura
After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo.
