"
Team:Paris Liliane Bettencourt/Notebook/2011/07/12/
From 2011.igem.org
(Difference between revisions)
LauradaSilva (Talk | contribs) |
|||
| (2 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | Kevin | + | {{:Team:Paris_Bettencourt/tpl_test}} |
| + | |||
| + | ==Kevin== | ||
'''Plasmid cultures from David Lane (LacO/tetO array)''' <br> | '''Plasmid cultures from David Lane (LacO/tetO array)''' <br> | ||
-10mL LB + 10μL Antibiotics overnight<br><br> | -10mL LB + 10μL Antibiotics overnight<br><br> | ||
| - | -pDAG480 -> CmR - 1<br> | + | -pDAG480 -> CmR - 1 - tetO array<br> |
| - | -pDAG479 -> CmR - 2 <br> | + | -pDAG479 -> CmR - 2 - tetO array<br> |
| - | -pFX234 -> Amp - 3 <br> | + | -pFX234 -> Amp - 3 - yfp:tetR<br> |
| - | -pDAG464 -> CmR , KanR - 4 <br> | + | -pDAG464 -> CmR , KanR - 4 - lacO array<br> |
| - | -pSG20 -> Amp - 5 <br> | + | -pSG20 -> Amp - 5 - gfp:lacI<br> |
| + | |||
| + | ==Mathias & Laura== | ||
| + | |||
| + | After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo. | ||
Latest revision as of 15:53, 21 September 2011
Kevin
Plasmid cultures from David Lane (LacO/tetO array)
-10mL LB + 10μL Antibiotics overnight
-pDAG480 -> CmR - 1 - tetO array
-pDAG479 -> CmR - 2 - tetO array
-pFX234 -> Amp - 3 - yfp:tetR
-pDAG464 -> CmR , KanR - 4 - lacO array
-pSG20 -> Amp - 5 - gfp:lacI
Mathias & Laura
After sequencing, we could see the BioBrick the parts registry sent us under the name K143055 was not pHyperSpank + SpoVg but pVeg + SpoVg we have to all redo.
