Team:Wageningen UR/Project/PartsProj1

From 2011.igem.org

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(→Synchroscillator: Data page/parts submitted to the registry)
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=== Parts Submitted to the Registry ===
=== Parts Submitted to the Registry ===
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[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - '''3OC6HSL Receiver Device with lux pR:''' 3-oxohexanoyl-homoserine lactone (AHL) responsive element that constitutively produces an intermediate level of LuxR via lux pL promoter. Combined with AHL, luxR activates lux pR promoter.
 
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[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - '''3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene:''' AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.  
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[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - '''3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene:''' AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.
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===Data for pre-existing parts===
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[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - '''3OC6HSL Receiver Device with lux pR:''' 3-oxohexanoyl-homoserine lactone (AHL) responsive element that constitutively produces an intermediate level of LuxR via lux pL promoter. Combined with AHL, luxR activates lux pR promoter. This part is a repaired version of [http://partsregistry.org/Part:BBa_F2621 BBa_F2621] that appeared to be defective (R0063 missing), when we sequenced the part.
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Revision as of 12:17, 21 September 2011

Building a Synchronized Oscillatory System

Synchroscillator: Data page/parts submitted to the registry

Parts Submitted to the Registry

[http://partsregistry.org/Part:BBa_K546002 BBa_K546002] - 3OC6HSL Receiver Device with a lux pR regulated GFP (including a LVA Tag) Reporter: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of GFP. Upon addition of AHL the GFP expression increases.


[http://partsregistry.org/Part:BBa_K546000 BBa_K546000] - Autoinducer 3OC6HSL Receiver Device with a lux pR Controlled Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase.


[http://partsregistry.org/Part:BBa_K546005 BBa_K546005] - Autoinducer 3OC6HSL Receiver Device with a lux pR Regulated GFP (including a LVA Tag) & Autoinducer Synthetase: AHL responsive element that produces a low constitutive level of luxR via lux pL. Combined with AHL, luxR induces lux pR. lux pR regulates the expression of autoinducer synthetase. The system creates a positive feedback loop, yielding high titres of GFP.


[http://partsregistry.org/Part:BBa_K546001 BBa_K546001] - 3OC6HSL Receiver Device with a regulated 3OC6HSL inactivation enzyme gene: AHL responsive element that produces a low constitutive level of luxR via pL. Combined with 3-oxohexanoyl-homoserine lactone, luxR induces pR. lux pR regulates the autoinducer inactivation enzyme A (aiiA) gene that degrades AHL.


Data for pre-existing parts

[http://partsregistry.org/Part:BBa_K546003 BBa_K546003] - 3OC6HSL Receiver Device with lux pR: 3-oxohexanoyl-homoserine lactone (AHL) responsive element that constitutively produces an intermediate level of LuxR via lux pL promoter. Combined with AHL, luxR activates lux pR promoter. This part is a repaired version of [http://partsregistry.org/Part:BBa_F2621 BBa_F2621] that appeared to be defective (R0063 missing), when we sequenced the part.

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