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Team:Freiburg/Notebook/4 September
From 2011.igem.org
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No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones | No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones | ||
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==<span style="color:orange;">Lysis cassette</span>== | ==<span style="color:orange;">Lysis cassette</span>== | ||
Revision as of 12:12, 21 September 2011
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Contents |
green light receptor
transformation
Investigators:Theo
competent E.coli were transformed with 5mikroiter Ligation reaction from 3 September
blue light receptor
picking clones
Investigators:Sophie
No clone of the NOT-psB1C3-ligation grew. The ♥-psB1c3-ligation produced only 2 clones
Lysis cassette
3A Assembly of Quickchange-modified K098995 + modified Lysis genes K124017
Investigators:Theo
Another miniprep of the parts was done
- S66 = modified Lysis genes K124017 in pSB1A2
- GFP = E0040 in pSB1A2
- S48 = Quickchange-modified K098995 in pSB1A2
- S39 = Promotor (J23104) + RBS (B0034) in pSB1C3
As we thought, there is no band for S66, although there is DNA isolated from the mini-prep. It is probably a matter of making a stock from the false mini-prep. More answers on Monday or Tuesday
Precipitator
NAME OF YOUR EXPERIMENT
Investigators: NAME
