Team:KULeuven/Data

From 2011.igem.org

(Difference between revisions)
Line 136: Line 136:
<br><br>
<br><br>
<h2> Data for pre-existing parts</h2>
<h2> Data for pre-existing parts</h2>
-
<li> 1. <b>BBa_I13453</b> <a href="http://partsregistry.org/Part:BBa_I13453:Experience#User_Reviews" target="blank"> (Experience) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.<br><br>
+
<li> 1. <b>BBa_I13453</b> <a href="http://partsregistry.org/Part:BBa_I13453:Experience#User_Reviews" target="blank"> (Experience) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.
-
<li> 2. <b>BBa_J23119</b> <a href="http://partsregistry.org/Part:BBa_J23119:Experience" target="blank"> (Experience) </a> - <b> constitutive promoter</b>, <b> BBa_J23119 </b> (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584001" target="blank"> BBa_K584001</a><br><br>
+
<br><br>
 +
<li> 2. <b>BBa_J23119</b> <a href="http://partsregistry.org/Part:BBa_J23119:Experience" target="blank"> (Experience) </a> - <b> constitutive promoter</b>, <b> BBa_J23119 </b> (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584001" target="blank"> BBa_K584001</a>). The results can be found at the experience page of that promoter.
 +
<br><br>
<h2>We've also characterized the following parts</h2>
<h2>We've also characterized the following parts</h2>

Revision as of 10:01, 21 September 2011

KULeuven iGEM 2011

close

Data page



How does E.D. Frosti work?



When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.



Data for our favorite new parts

  • 1. BBa_K584028 (Main Page) - pLac-Lux hybrid promoter + INP , BBa_K584028 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. We added 1mM IPTG to E.D. Frosti's medium. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing.

  • 2. BBa_K584027 (Main Page) - Constitutive Promoter + INP , BBa_K584027 (K.U.Leuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition. Addition of a control (GFP construct) E.D. Frosti did not result in freezing.

  • 3. BBa_K584026 (Main Page) - constitutive promoter + AFP , BBa_K584026 (K.U.Leuven, iGEM 2011): We used the same conditions for the MilliQ water, but now at -10°C. Unfortunately addition of E.D. Frosti coated with AFP (or nothing: GFP control) did not result in prevention of ice formation. Due to lack of time we could not test other conditions (different temperatures, different concentrations,...)

    Data for pre-existing parts

  • 1. BBa_I13453 (Experience) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.

  • 2. BBa_J23119 (Experience) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001). The results can be found at the experience page of that promoter.

    We've also characterized the following parts

  • 1. BBa_K584004 (Main Page) - HybB promoter + GFP generator, BBa_K584004 (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.


    • Retrieved from "http://2011.igem.org/Team:KULeuven/Data"