Latest revision as of 09:55, 21 September 2011

DNA Recovery Experiment

Once the basic mechanism of cell lysing is confirmed, the next step is to show that DNA can be recovered from the supernatant.

The Experiment : The Experimental Setup for the Lysis-based DNA Recovery Experiment

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 * [[Team:EPF-Lausanne/Our Project/T7 promoter variants/dnarecov/results| The Results ]]: The Results from the Lysis-based DNA Recovery Experiment
- We grow two large cultures of cells. One contains cells that will lyse and release plasmids into the supernatant while the other has non-lysing, "normal" cells.
-[[File:broth_noiptg.png]]
-Adding IPTG to both flasks induces lysis in one set of cells but not in the others.
-[[File:broth_iptg_induction.png]]
-Thanks to qPCR, the supernatant harvested from the lysing culture reveals increased numbers of plasmids while the non-lysing culture exhibits significantly lower numbers of plasmids. A similar test involves transforming the supernatant (whose plasmid content is not known a priori) into cells and counting the number of resulting colonies. The plates containing transformations from the lysing supernatant have vastly superior number of colonies compared to the non-lysing supernatant transformations.
-[[File:iptg_platecount_text.png|700px]]
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