Team:KULeuven/Data

From 2011.igem.org

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<h2>Data for our favorite new parts</h2>
<h2>Data for our favorite new parts</h2>
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<li>1. <b>Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> (link) </a> - <b> Constitutive Promoter + INP </b>, <b> BBa_K584027 </b> (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Addition
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<li> 1. <b>Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584028" target="blank"> (link) </a> - <b> pLac-Lux hybrid promoter + INP </b>, <b> BBa_K584028 </b> (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. 2 tests were performed: 1mM or 100mM were added to <i>E.D. Frosti</i>'s medium. Water freezes immediately upon addition in both cases.
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<br><br>
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-6°C en gewerkt MilliQ (gedenateraliseerd water) gefilterd
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<li> 2. <b>Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584027" target="blank"> (link) </a> - <b> Constitutive Promoter + INP </b>, <b> BBa_K584027 </b> (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition.
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via reversed osmosis over 0,45 mM filter en nadien 0,20mM
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<br><br>
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om alle nucleatie agents weg te krijgen.<br><br>
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<li> 3. <b>Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584026" target="blank"> (link) </a> - <b> constitutive promoter + AFP </b>, <b> BBa_K584026 </b> (KULeuven, iGEM 2011): test
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<br><br>
<h2> Data for pre-existing parts</h2>
<h2> Data for pre-existing parts</h2>
<li> 1. <b>Experience</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453:Experience" target="blank"> (link) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.<br><br>
<li> 1. <b>Experience</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_I13453:Experience" target="blank"> (link) </a> - <b> pBAD promoter</b>, <b> BBa_I13453 </b> (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584000" target="blank"> <b>BBa_K584000</b></a>). The results can be found at the experience page of that promoter.<br><br>
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<h2>We've also characterized the following parts</h2>
<h2>We've also characterized the following parts</h2>
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<li> 1. <b>Main page</b> <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K584004" target="blank"> (link) </a> - <b> HybB promoter + GFP generator</b>, <b> BBa_K584004 </b> (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.
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Revision as of 09:03, 21 September 2011

KULeuven iGEM 2011

close

Data page



How does E.D. Frosti work?



When Lactose is added to the medium, plasmid 1 will be induced to form INP via BBa_K584028.
When L-arabinose is added to the medium, plasmid 2 will be induced to form AFP via.



Data for our favorite new parts

  • 1. Main page (link) - pLac-Lux hybrid promoter + INP , BBa_K584028 (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. 2 tests were performed: 1mM or 100mM were added to E.D. Frosti's medium. Water freezes immediately upon addition in both cases.

  • 2. Main page (link) - Constitutive Promoter + INP , BBa_K584027 (KULeuven, iGEM 2011): Tested with MilliQ water (first purified via reversed osmosis over 0,45 µm then filtered through a 0,22µm filter to remove all nucleating agents) at -6°C. Water freezes immediately upon addition.

  • 3. Main page (link) - constitutive promoter + AFP , BBa_K584026 (KULeuven, iGEM 2011): test

    Data for pre-existing parts

  • 1. Experience (link) - pBAD promoter, BBa_I13453 (Endy Lab, iGEM 2005): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584000). The results can be found at the experience page of that promoter.

  • 2. Experience (link) - constitutive promoter, BBa_J23119 (Berkeley, iGEM 2006): Fused a GFP reporter to this promoter (which created biobrick: BBa_K584001

    We've also characterized the following parts

  • 1. Main page (link) - HybB promoter + GFP generator, BBa_K584004 (KULeuven, iGEM 2011): We created this biobrick but due to lack of time we could not test it.


    • Retrieved from "http://2011.igem.org/Team:KULeuven/Data"