Team:Lyon-INSA-ENS/Project/Parts

From 2011.igem.org

(Difference between revisions)
 
(8 intermediate revisions not shown)
Line 9: Line 9:
<html>
<html>
-
<style type="text/css";>
+
 
-
.firstHeading{background-image: url(https://static.igem.org/mediawiki/2011/7/7f/Banniere_realisation.png);background-repeat: no repeat;}
+
-
    </style>
+
<style type="text/css">
<style type="text/css">
Line 32: Line 30:
</style>
</style>
 +
 +
<SCRIPT LANGUAGE="javascript">
 +
 +
Image1 = new Image(650,479)
 +
Image1.src = "https://static.igem.org/mediawiki/2011/e/eb/Schema_projet.png"
 +
 +
Image2 = new Image(650,479)
 +
Image2.src = "https://static.igem.org/mediawiki/2011/2/28/Schema_etape_1.png"
 +
 +
Image3 = new Image(650,479)
 +
Image3.src = "https://static.igem.org/mediawiki/2011/2/20/Schema1_Etape2.jpg"
 +
 +
Image4 = new Image(650,479)
 +
Image4.src = "https://static.igem.org/mediawiki/2011/f/ff/Schema1_Etape3.jpg"
 +
 +
Image5 = new Image(650,479)
 +
Image5.src = " https://static.igem.org/mediawiki/2011/9/92/Schema1_etape4.jpg"
 +
 +
Image6 = new Image(650,479)
 +
Image6.src = "https://static.igem.org/mediawiki/2011/4/45/Schema1_etape5.jpg"
 +
 +
Image7 = new Image(650,479)
 +
Image7.src = "https://static.igem.org/mediawiki/2011/1/19/Schema2.jpg"
 +
 +
Image8 = new Image(650,479)
 +
Image8.src = "https://static.igem.org/mediawiki/2011/4/43/Schema2_phase1.jpg"
 +
 +
Image9 = new Image(650,479)
 +
Image9.src = "https://static.igem.org/mediawiki/2011/f/f3/Schema2_phase2.jpg"
 +
 +
Image10 = new Image(650,479)
 +
Image10.src = "https://static.igem.org/mediawiki/2011/9/9d/Schema2_phase3.jpg"
 +
 +
</SCRIPT>
 +
 +
<SCRIPT LANGUAGE="javascript">
 +
 +
function etape1() {
 +
document.emp.src = Image2.src; return true;
 +
}
 +
 +
function etape2() {
 +
document.emp.src = Image3.src; return true;
 +
}
 +
 +
function etape3() {
 +
document.emp.src = Image4.src; return true;
 +
}
 +
 +
function etape4() {
 +
document.emp.src = Image5.src; return true;
 +
}
 +
 +
function etape5() {
 +
document.emp.src = Image6.src; return true;
 +
}
 +
 +
function original() {
 +
document.emp.src = Image1.src; return true;
 +
}
 +
 +
function original2() {
 +
document.emp2.src = Image7.src; return true;
 +
}
 +
 +
function etape1_2() {
 +
document.emp2.src = Image8.src; return true;
 +
}
 +
 +
function etape2_2() {
 +
document.emp2.src = Image9.src; return true;
 +
}
 +
 +
function etape3_2() {
 +
document.emp2.src = Image10.src; return true;
 +
}
 +
 +
</SCRIPT>
<div style="float : left; margin-top : -10px; margin-left : -200px">
<div style="float : left; margin-top : -10px; margin-left : -200px">
Line 42: Line 118:
<body>
<body>
<div id="language";>
<div id="language";>
-
         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/IntroductionFr">Version Française</a>
+
         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/PartFr">Version Française</a>
     </div>
     </div>
 +
  <map name="ma_map" id="id_map">
 +
      <area shape="rect" coords="6,35,55,80" alt="Stage 1" href="https://static.igem.org/mediawiki/2011/2/28/Schema_etape_1.png"  onmouseover="etape1()" onmouseout="original()"/> 
 +
      <area shape="rect" coords="60,183,114,232" alt="Stage 2" href="https://static.igem.org/mediawiki/2011/2/20/Schema1_Etape2.jpg"  onmouseover="etape2()" onmouseout="original()"/> 
 +
    <area shape="rect" coords="294,135,350,185" alt="Stage 3" href="https://static.igem.org/mediawiki/2011/f/ff/Schema1_Etape3.jpg"  onmouseover="etape3()" onmouseout="original()"/> 
 +
    <area shape="rect" coords="558,271,604,313" alt="Stage 4" href="https://static.igem.org/mediawiki/2011/9/92/Schema1_etape4.jpg"  onmouseover="etape4()" onmouseout="original()"/> 
 +
    <area shape="rect" coords="125,388,178,434" alt="Stage 5" href="https://static.igem.org/mediawiki/2011/4/45/Schema1_etape5.jpg"  onmouseover="etape5()" onmouseout="original()"/> 
 +
  </map>
 +
 +
  <map name="ma_map2" id="id_map2">
 +
      <area shape="rect" coords="224,365,282,415" alt="Stage 1" href="https://static.igem.org/mediawiki/2011/4/43/Schema2_phase1.jpg" onmouseover="etape1_2()" onmouseout="original2()"/>
 +
      <area shape="rect" coords="196,146,242,203" alt="Stage 2" href="https://static.igem.org/mediawiki/2011/f/f3/Schema2_phase2.jpg" onmouseover="etape2_2()" onmouseout="original2()"/>
 +
      <area shape="rect" coords="45,255,100,304" alt="Stage 3" href="https://static.igem.org/mediawiki/2011/9/9d/Schema2_phase3.jpg" onmouseover="etape3_2()" onmouseout="original2()"/>
 +
  </map>
 +
 +
<div class="contenugrand2";>
<div class="contenugrand2";>
 +
 +
<br><br><br/><br/>
 +
      <p id="top"> <font color="green" size="6">
 +
              Data page <br><HR>
 +
              <br/>
 +
          </font>
 +
      </p>
 +
 +
 +
<ul style="list-style-type:circle;margin-left:10%;">       
 +
              <li> <a href="#rcn-csgBAEFG"> <font color="green"> <b style="line-height : 1.5em"> Overproduction of curli via a synthetic operon controled by a cobalt-inducible promoter P<i>rcn-csgBAEFG</i> </b> </font> </a> </li>
 +
              <br/>
 +
      <li> <a href="#ompR"> <font color="green"> <b>Overexpression of curli genes via the superactivator OmpR234  </b> </font> </a> </li>
 +
              <br>
 +
              <li> <a href="#parts"> <font color="green"> <b> Our parts </b> </font> </a> </li>
 +
              <br/>
 +
        </ul>
<br/><br/><br/>
<br/><br/><br/>
-
<p> <font color="green" size="5">
+
 
-
               Rcn-csgBAEFG : </font><font color="green" size="4"> curli under control of the cobalt-inducible promoter<br><HR>
+
        <p id="rcn-csgBAEFG"> <font color="green" size="4" style="line-height : 1.5em">
 +
               <big>Overproduction of curli </big>via a synthetic operon controled by a cobalt-<br>inducible promoter P<i>rcn-csgBAEFG</i><br><HR>
 +
          </font></p>
 +
 
 +
<br> <br>
 +
<p>
 +
Hover the mouse over the numbers to see the stages in detail.
 +
<br> <br>
 +
</p>
 +
   
 +
<div style="text-align:center;">
 +
<img name="emp" src="https://static.igem.org/mediawiki/2011/e/eb/Schema_projet.png" heigth="479px" width="650px" border=0 usemap="#ma_map"/>
 +
</div>
 +
 
 +
<br><br><br><br><br>
 +
 
 +
        <p id="ompR"> <font color="green" size="4">
 +
              <big>Overexpression of curli genes</big> via the superactivator OmpR234<br><HR>
           </font></p>
           </font></p>
<br> <br><br> <br>
<br> <br><br> <br>
 +
<p>
 +
Hover the mouse over the numbers to see the stages in detail.
 +
<br> <br>
 +
</p>
 +
   
      
      
<div style="text-align:center;">
<div style="text-align:center;">
-
<img src="https://static.igem.org/mediawiki/2011/4/42/Rcncsg_mecanisme_schema.jpg"/ heigth="350px" width="700px">
+
<img name="emp2" src="https://static.igem.org/mediawiki/2011/1/19/Schema2.jpg" heigth="479px" width="650px" border=0 usemap="#ma_map2"/>
</div>
</div>
-
<br/> <br/>
+
<br><br>
 +
<br>
 +
<br>
     <br/><br/><br/><br/><br/>
     <br/><br/><br/><br/><br/>
-
         <p> <font color="green" size="6">
+
         <p id="parts"> <font color="green" size="6">
               Our parts<br><HR>
               Our parts<br><HR>
           </font></p>
           </font></p>

Latest revision as of 07:39, 21 September 2011




Stage 1 Stage 2 Stage 3 Stage 4 Stage 5 Stage 1 Stage 2 Stage 3




Data page






Overproduction of curli via a synthetic operon controled by a cobalt-
inducible promoter Prcn-csgBAEFG




Hover the mouse over the numbers to see the stages in detail.






Overexpression of curli genes via the superactivator OmpR234






Hover the mouse over the numbers to see the stages in detail.










Our parts





Data for our favorite new parts

1.Main page - rcn-csgBAEFG, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.
2.Main page - rcn, cobalt-inducible promoter, BBa_K540001 . This promoter increases the production of a gfp placed under its control when cobalt is in the medium.



Data for pre-existing parts

1.Main page - ompR234, a stimulating gene for curli production, BBa_K342003 This part has been shown to induce adherence as well.












ENS assystem Biomérieux INSA INSA