Team:Lyon-INSA-ENS/Project/Parts

From 2011.igem.org

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<div style="float : left; margin-top : -10px; margin-left : -200px">
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  <a href="https://2011.igem.org/Main_Page" >
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      <img src="https://static.igem.org/mediawiki/2011/6/67/Team_INSA-Lyon_IGEM_Home.png" title="iGEM's main page" />
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<body>
<body>
<div id="language";>
<div id="language";>
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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Realisation/ExperimentFr">Version Française</a>
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         <img src="https://static.igem.org/mediawiki/2011/0/0e/Drapeau_francais.jpg"; width=20px; /> <a href="https://2011.igem.org/Team:Lyon-INSA-ENS/Project/PartFr">Version Française</a>
     </div>
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  <map name="ma_map" id="id_map">
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<div class="contenugrand2";>
<div class="contenugrand2";>
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<br/> <br/>
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<br><br><br/><br/>
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    <br/><br/><br/>
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      <p id="top"> <font color="green" size="6">
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              Data page <br><HR>
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              <br/>
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          </font>
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      </p>
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         <p> <font color="green" size="5">
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               Our parts<br><HR>
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<ul style="list-style-type:circle;margin-left:10%;">          
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          </font></p>
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              <li> <a href="#rcn-csgBAEFG"> <font color="green"> <b style="line-height : 1.5em"> Overproduction of curli via a synthetic operon controled by a cobalt-inducible promoter P<i>rcn-csgBAEFG</i> </b> </font> </a> </li>
 +
               <br/>
 +
      <li> <a href="#ompR"> <font color="green"> <b>Overexpression of curli genes via the superactivator OmpR234  </b> </font> </a> </li>
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              <br>
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              <li> <a href="#parts"> <font color="green"> <b> Our parts </b> </font> </a> </li>
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              <br/>
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        </ul>
<br/><br/><br/>
<br/><br/><br/>
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<p style="line-height:1.5em">
 
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<b>Data for our favorite new parts </b> <br/><br/>
 
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1.<a href="http://partsregistry.org/Part:BBa_K540000">Main page</a> - <b>rcn-csgBAEFG, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000</b>. This part enable production of curli induced cobalt in the medium.<br/>
 
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2.<a href="http://partsregistry.org/Part:BBa_K540001">Main page</a> - <b>rcn, cobalt-inducible promoter, BBa_K540001 </b>. This promoter makes the expression of the following gene cobalt-dependent.<br/>
 
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3.<a href="http://partsregistry.org/Part:BBa_K540003">Main page</a> - <b>rcn-OmpR234, cobalt-inducible promoter controlling a stimulating gene for curli production, BBa_K540003 </b>. This part produces curli when cobalt is in the medium, as rcn is cobalt-dependent promoter, and OmpR234 activates the csg genes.
 
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</p>
 
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<br/><br/>
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        <p id="rcn-csgBAEFG"> <font color="green" size="4" style="line-height : 1.5em">
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<p style="line-height:1.5em">
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              <big>Overproduction of curli </big>via a synthetic operon controled by a cobalt-<br>inducible promoter P<i>rcn-csgBAEFG</i><br><HR>
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<b>Data for pre-existing parts </b> <br/><br/>
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          </font></p>
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1.<a href="http://partsregistry.org/Part:BBa_K342003">Main page</a> - <b>OmpR234, a stimulating gene for curli production, BBa_K342003 </b><br/>
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 +
<br> <br>
 +
<p>
 +
Hover the mouse over the numbers to see the stages in detail.
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<br> <br>
</p>
</p>
 +
   
 +
<div style="text-align:center;">
 +
<img name="emp" src="https://static.igem.org/mediawiki/2011/e/eb/Schema_projet.png" heigth="479px" width="650px" border=0 usemap="#ma_map"/>
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</div>
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<br/><br/>
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<br><br><br><br><br>  
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<p style="line-height:1.5em">
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<b>We've also characterized the following parts </b> <br/><br/>
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1.<a href="http://partsregistry.org/Part:BBa_K540002">Main page</a> - <b>Ptrc, a strong promoter, BBa_K540003 </b><br/>
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  </p>
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<br/> <br/>
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         <p id="ompR"> <font color="green" size="4">
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    <br/><br/><br/><br/><br/>
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               <big>Overexpression of curli genes</big> via the superactivator OmpR234<br><HR>
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         <p> <font color="green" size="5">
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               Rcn-csgBAEFG : placing the curli secretion under control of the cobalt-inducible promoter<br><HR>
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           </font></p>
           </font></p>
<br> <br><br> <br>
<br> <br><br> <br>
 +
<p>
 +
Hover the mouse over the numbers to see the stages in detail.
 +
<br> <br>
 +
</p>
 +
   
      
      
<div style="text-align:center;">
<div style="text-align:center;">
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<img src="https://static.igem.org/mediawiki/2011/4/42/Rcncsg_mecanisme_schema.jpg"/ heigth="350px" width="700px">
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<img name="emp2" src="https://static.igem.org/mediawiki/2011/1/19/Schema2.jpg" heigth="479px" width="650px" border=0 usemap="#ma_map2"/>
</div>
</div>
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<br/> <br/>
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<br><br>
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<br>
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<br>
     <br/><br/><br/><br/><br/>
     <br/><br/><br/><br/><br/>
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         <p> <font color="green" size="5">
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         <p id="parts"> <font color="green" size="6">
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               Rcn-OmpR234 : Stimulating the chromosomal curli secretion genes with the cobalt-inducible promoter<br><HR>
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               Our parts<br><HR>
           </font></p>
           </font></p>
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<br> <br><br> <br>
+
<br/><br/><br/>
 +
 
 +
<p style="line-height:1.5em">
 +
<b>Data for our favorite new parts </b> <br/><br/>
 +
1.<a href="http://partsregistry.org/Part:BBa_K540000">Main page</a> - <b><i>rcn-csgBAEFG</i>, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000</b>. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.<br/>
 +
2.<a href="http://partsregistry.org/Part:BBa_K540001">Main page</a> - <b><i>rcn</i>, cobalt-inducible promoter, BBa_K540001 </b>. This promoter increases the production of a gfp placed under its control when cobalt is in the medium.<br/>
 +
</p>
 +
 
 +
<br/><br/>
 +
<p style="line-height:1.5em">
 +
<b>Data for pre-existing parts </b> <br/><br/>
 +
1.<a href="http://partsregistry.org/Part:BBa_K342003">Main page</a> - <b><i>ompR234</i>, a stimulating gene for curli production, BBa_K342003 </b> This part has been shown to induce adherence as well.<br/>
 +
</p>
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 +
 
 +
<br/> <br/>
 +
    <br/><br/><br/><br/><br/>
 +
 
-
<div style="text-align:center;">
 
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<img src="https://static.igem.org/mediawiki/2011/1/1a/RcnompR_mecanisme_schema.jpg"/ heigth="350px" width="700px">
 
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</div> 
 

Latest revision as of 07:39, 21 September 2011




Stage 1 Stage 2 Stage 3 Stage 4 Stage 5 Stage 1 Stage 2 Stage 3




Data page





Overproduction of curli via a synthetic operon controled by a cobalt-
inducible promoter Prcn-csgBAEFG



Hover the mouse over the numbers to see the stages in detail.






Overexpression of curli genes via the superactivator OmpR234





Hover the mouse over the numbers to see the stages in detail.










Our parts




Data for our favorite new parts

1.Main page - rcn-csgBAEFG, cobalt-inducible promoter controlling a curli-generating gene, BBa_K540000. This part enable production of cobalt induced curlis, which induces the formation of a biofilm.
2.Main page - rcn, cobalt-inducible promoter, BBa_K540001 . This promoter increases the production of a gfp placed under its control when cobalt is in the medium.



Data for pre-existing parts

1.Main page - ompR234, a stimulating gene for curli production, BBa_K342003 This part has been shown to induce adherence as well.












ENS assystem Biomérieux INSA INSA
Retrieved from "http://2011.igem.org/Team:Lyon-INSA-ENS/Project/Parts"