Team:Paris Liliane Bettencourt/Notebook/2011/09/20/

From 2011.igem.org

(Difference between revisions)
(Cyrille)
 
(4 intermediate revisions not shown)
Line 8: Line 8:
[[File:CP2009.jpg|thumb|center]]
[[File:CP2009.jpg|thumb|center]]
 +
Control of the size of the insert
-
 
+
[[File:CP2009_XP.jpg|thumb|center|S75 *5; S82 *5; 104 *5]]
-
 
+
[[File:CP2009_XP_ComS.jpg|thumb|center|ComS *3]]
== Baptiste, Hovannes & Ouriel ==
== Baptiste, Hovannes & Ouriel ==
Line 22: Line 23:
{| border="1" class="wikitable" style="text-align: center;"
{| border="1" class="wikitable" style="text-align: center;"
-
|+T7 autoloop RFP+ Control in E-coli at 37°C
+
|+T7 autoloop RFP+ Control / E-coli at 37°C
|-
|-
|[[File:T7 autoloop ctrl-7.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
|[[File:T7 autoloop ctrl-7.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
Line 32: Line 33:
|[[File:T7 autoloop ctrl-12.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
|[[File:T7 autoloop ctrl-12.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
|}
|}
-
 
-
===YFP-tetR===
 
-
 
-
We
 
-
 
-
 
Line 44: Line 39:
{| border="1" class="wikitable" style="text-align: center;"
{| border="1" class="wikitable" style="text-align: center;"
-
|+T7 autoloop RFP+ Control in E-coli at 37°C
+
|+T7 autoloop full no terminator / E-coli at 37°C
|-
|-
|[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
|[[File:T7 autoloop ctrl-25.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
Line 54: Line 49:
|[[File:T7 autoloop ctrl-33.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
|[[File:T7 autoloop ctrl-33.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
|}
|}
 +
 +
 +
{| border="1" class="wikitable" style="text-align: center;"
 +
|+T7 autoloop full with  terminator / E-coli at 37°C
 +
|-
 +
|[[File:T7_loop_1.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
 +
|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
 +
|[[File:T7 loop 1 rfp.jpg|290px|thumb|center|E-Coli  at 37°C  (rfp image)]]
 +
|-
 +
|[[File:T7 loop 2.jpg|290px|thumb|center|E-Coli  at 37°C  (trans image)]]
 +
|[[File:T7 loop 1 gfp.jpg|290px|thumb|center|E-Coli  at 37°C  (gfp image)]]
 +
|[[File:T7 loop 2 rfp.jpg|290px|thumb|center|-Coli  at 37°C  (rfp image)]]
 +
|}
 +
 +
===YFP-tetR===
 +
 +
We

Latest revision as of 21:01, 20 September 2011

Team IGEM Paris 2011


Contents

Cyrille

Control digestion of pHM3 in NcoI

CP2009.jpg

Control of the size of the insert

S75 *5; S82 *5; 104 *5
ComS *3

Baptiste, Hovannes & Ouriel

Characterisation of T7 autoloop, RFP - GFP

We characterized T7 autoloop by microscopy. Leak,


T7 autoloop RFP+ Control / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


TEXT


T7 autoloop full no terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)


T7 autoloop full with terminator / E-coli at 37°C
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
E-Coli at 37°C (rfp image)
E-Coli at 37°C (trans image)
E-Coli at 37°C (gfp image)
-Coli at 37°C (rfp image)

YFP-tetR

We


TEXT