Team:Bilkent UNAM Turkey/Team

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Ass.Prof. TT EK AET ADO BG SG TTO UD

Biodegradation of TNT and TNT derivatives by nfsI-transfected Chlamydomonas Reinhardtii

Abstract

We aim for the genetic modification of the unicellular microalga Chlamydomonas reinhardtii by introducing the nfsI gene belonging to the bacterium Enterobacter cloacae in order to investigate how nitroreductase expressing-microalgae respond to trinitrotoluene (TNT) exposure. Our experimental design is as follows: firstly, obtain a synthetic gene of nfsI with flanking prefix and suffix of standard Biobricks, and ligate this insert to pRbcBRL, a vector with appropriate expression and selection system for Chlamydomonas reinhardtii and obtain pRbcnfsI. Then, Chlamydomonas reinhardtii will be transfected with the with the designed plasmid. The transfected algae will then be grown in presence of TNT and/or TNT derivatives and the effectiveness of nitroreductase activity on biological degradation of TNT will be investigated.

I’m Ebuzer, graduated from the Middle East Technical University and I’ am starting my PhD in UNAM. My hobbies consist of football, squash, Dota, watching movies, pen spinning. My favourites are shawshank redemption (movie), Game of thrones (book), Dexter (series). At last I am really interested in biodiesel production and algal systems. This year we got a grateful team, working hard and getting fun are our basic rules.

I’m Ufuk, sophomores of Bilkent University, Molecular Biology and Genetics Department. Playing squash and Dota are my favourite activities, if I win. Fantastic books are fascinates me. First book, I read was One Thousand and One Nights. In iGEM; I like its fun and working style. Everything happens together. I like to watch movies especially with friends. This is my first iGEM experience, I am thankful to my team members for sharing their fun.

I am Tarkan and one of the member of the Bilkent – UNAM – Turkey team. I love watching and playing sports especially football and basketball just as usual Turkish guys do. I love dancing but generally too shy to start doing it in public. I like talking and meeting new people. Telling jokes and amuzing people is my style of communication, because that is the best way of telling your good intentions towards others. I participate volunteering action a lot in the campus and that actions target poor, rural and disabled children. I love them and enjoy spending time with. So, that is it for me now.
It is Burcu from Bilkent University, Institute of Material Science and Nanotechnology. I am very proud of being a member of Bilkent UNAM Turkey team because I think; here we reflect the real team spirit. Here is a brief information about me: I spent every minute of me by listening music! Nothing makes me happy like talking with people. I do love sports activities if I can bring down my laziness. Reading books is not for me but reading articles has become my pleasure for a long time. As many members of my team, it is my first IGEM participation. I hope we will rock the IGEM world with our endless energy and success!

In this summer; we learned about iGEM, how it works, what the biggest part of competition is and how we can win a medallion.

When we were newbie, we organized a lot of meeting to find out our main project than we got idea for improving human welfare so we choose an idea from brainstorm results. We searched to get a gene to work on. As a result; lab works has began. We added our protocols which are helpful to transform C.reinhartii and E.coli. Basic cloning protocols also added our protocol page. Experiments and safety questions also available from link at the top section.

IGEM Project is our number one priority. As we worked really hard for our project it is important for us to make our dreams come true which is taking a gold medal. Questioning different items like experiment time, materials and methods and the efficiency of the experiments improved our skills and we started to look as a scientist to problems we faced. There were lots of challenging parts and sometimes we thought to quit but we listened to “Eye of The Tiger” and we did not give up from our experiments we delayed them yes, but we never gave up.
IGEM team- meet-up was a great opportunity for us to communicate with the other groups, understood their projects and share our ideas. As we had a chance for an interview with Anatolia Agency, we also used media power to get attention to our project, iGEM and the importance of synthetic biology.
Apart from that we did not forget having fun apart from our lab. As we planned a BBQ party, it was also a great chance for us to relax and gain some energy for our experiments, I did not even mention about the delicious foods and how joyful was our activities. Also like in iGEM team meeting up, we had a chance to introduce our project and main aim to other scientists and researchers in different areas.
Links at above lead you to specific event and its photos suppose to be at that link. If not, look at gallery link. Thanks and get great time.

Addded genes

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@@ Line 172: / Line 172: @@
 		<span class='tab_item'>
-			Turgay Tekinay
+			Ass.Prof. TT
@@ Line 178: / Line 178: @@
                 </span>
 		<span class='tab_item'>
-		       Ebuzer Kalyoncu
+		       EK
 	        </span>
 		<span class='tab_item'>
-		       Ahmet Emin Topal
+		       AET
                  </span>
 		<span class='tab_item'>
-		       Alper Devrim Ozkan
+		       ADO
 		</span>
 		<span class='tab_item'>
-		       Burcu Gumuscu
+		       BG
 		</span>
 		<span class='tab_item'>
-		       Sinan Gunes
+		       SG
 		</span>
 		<span class='tab_item'>
-		       Tolga Tarkan Olmez
+		       TTO
 		</span>
 		<span class='tab_item'>
-		       Ufuk Degirmenci
+		       UD
 		</span>