Team:LMU-Munich/Project

From 2011.igem.org

(Difference between revisions)
(→Project Abstract)
 
(7 intermediate revisions not shown)
Line 2: Line 2:
{{:Team:LMU-Munich/Templates/Page Header}}
{{:Team:LMU-Munich/Templates/Page Header}}
-
{|align="justify"
+
<div class="fullbox">
-
|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
+
== Project Abstract ==
-
|[[Image:LMU-Munich_logo.png|200px|right|frame]]
+
-
|-
+
-
|
+
-
''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
+
-
|[[Image:LMU-Munich_team.png|right|frame|Your team picture]]
+
-
|-
+
-
|
+
-
|align="center"|[[Team:LMU-Munich | Team Example]]
+
-
|}
+
-
<!--- The Mission, Experiments --->
+
Heavy metal ions of human origin contaminate waters worldwide and represent a major threat to human health, especially in lesser developed countries. The compliance with strict drinking water quality standards as a prerequisite to a healthy living requires qualitative and quantitative methods for monitoring metal ion concentration. Applying standard chemical methods is costly, complicated and sometimes requires high-tech machinery, which is often not available - especially where it's most urgently needed.
-
{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
+
We therefore aim at creating a set of bacterial biosensors for some of the most toxic heavy metal ions found in drinking water, by fusing metal-responsive promoters under the control of transcriptional regulators with reporter genes such as GFP. The biosensors will be evaluated to qualitatively determine the metal ion specificity and subsequently quantitatively describing the concentration-dependent output of the reporters. Such a tool kit can be applied to quantify the metal ion content in water samples in an easier and cheaper way.
-
!align="center"|[[Team:LMU-Munich|Home]]
+
-
!align="center"|[[Team:LMU-Munich/Team|Team]]
+
-
!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=LMU-Munich Official Team Profile]
+
-
!align="center"|[[Team:LMU-Munich/Project|Project]]
+
-
!align="center"|[[Team:LMU-Munich/Parts|Parts Submitted to the Registry]]
+
-
!align="center"|[[Team:LMU-Munich/Modeling|Modeling]]
+
-
!align="center"|[[Team:LMU-Munich/Notebook|Notebook]]
+
-
!align="center"|[[Team:LMU-Munich/Safety|Safety]]
+
-
!align="center"|[[Team:LMU-Munich/Attributions|Attributions]]
+
-
|}
+
 +
[[Team:LMU-Munich/Project/Description|Detailed information can be found here!]]
 +
</div>
-
==<font color="#9933CC">'''Overall project'''</font>==
 
-
Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated.
 
-
Bacteria sense metals in their surrounding in order to change their expression profile or react in order to adapt and accomodate to their environment.
+
<!-- Include the next line at the end of every page -->
-
Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output.
 
-
 
-
The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with a easy-to-see output is also needed.
 
-
 
-
In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water.
 
-
 
-
== Project Details==
 
-
 
-
 
-
 
-
 
-
 
-
=== Part 2 ===
 
-
 
-
 
-
 
-
 
-
 
-
=== The Experiments ===
 
-
 
-
 
-
 
-
 
-
=== Part 3 ===
 
-
 
-
 
-
 
-
 
-
== Results ==
 
-
 
-
<!-- Include the next line at the end of every page -->
 
{{:Team:LMU-Munich/Templates/Page Footer}}
{{:Team:LMU-Munich/Templates/Page Footer}}

Latest revision as of 17:01, 20 September 2011


Project Abstract

Heavy metal ions of human origin contaminate waters worldwide and represent a major threat to human health, especially in lesser developed countries. The compliance with strict drinking water quality standards as a prerequisite to a healthy living requires qualitative and quantitative methods for monitoring metal ion concentration. Applying standard chemical methods is costly, complicated and sometimes requires high-tech machinery, which is often not available - especially where it's most urgently needed.

We therefore aim at creating a set of bacterial biosensors for some of the most toxic heavy metal ions found in drinking water, by fusing metal-responsive promoters under the control of transcriptional regulators with reporter genes such as GFP. The biosensors will be evaluated to qualitatively determine the metal ion specificity and subsequently quantitatively describing the concentration-dependent output of the reporters. Such a tool kit can be applied to quantify the metal ion content in water samples in an easier and cheaper way.

Detailed information can be found here!




Retrieved from "http://2011.igem.org/Team:LMU-Munich/Project"