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| - | <!-- *** What falls between these lines is the Alert Box! You can remove it from your pages once you have read and understood the alert *** --> | + | <!-- Include the next line at the beginning of every page --> |
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| - | <div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;"> | + | == Project Abstract == |
| - | <div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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| - | This is a template page. READ THESE INSTRUCTIONS.
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| - | <div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You are provided with this team page template with which to start the iGEM season. You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki. You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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| - | <div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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| - | You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page. PLEASE keep all of your pages within your teams namespace.
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| + | Heavy metal ions of human origin contaminate waters worldwide and represent a major threat to human health, especially in lesser developed countries. The compliance with strict drinking water quality standards as a prerequisite to a healthy living requires qualitative and quantitative methods for monitoring metal ion concentration. Applying standard chemical methods is costly, complicated and sometimes requires high-tech machinery, which is often not available - especially where it's most urgently needed. |
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| + | We therefore aim at creating a set of bacterial biosensors for some of the most toxic heavy metal ions found in drinking water, by fusing metal-responsive promoters under the control of transcriptional regulators with reporter genes such as GFP. The biosensors will be evaluated to qualitatively determine the metal ion specificity and subsequently quantitatively describing the concentration-dependent output of the reporters. Such a tool kit can be applied to quantify the metal ion content in water samples in an easier and cheaper way. |
| - | |You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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| - | |[[Image:LMU-Munich_logo.png|200px|right|frame]]
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| - | ''Tell us more about your project. Give us background. Use this is the abstract of your project. Be descriptive but concise (1-2 paragraphs)''
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| - | |[[Image:LMU-Munich_team.png|right|frame|Your team picture]]
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| - | |align="center"|[[Team:LMU-Munich | Team Example]]
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| - | <!--- The Mission, Experiments --->
| + | [[Team:LMU-Munich/Project/Description|Detailed information can be found here!]] |
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| - | {| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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| - | !align="center"|[[Team:LMU-Munich|Home]]
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| - | !align="center"|[[Team:LMU-Munich/Team|Team]]
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| - | !align="center"|[https://igem.org/Team.cgi?year=2010&team_name=LMU-Munich Official Team Profile]
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| - | !align="center"|[[Team:LMU-Munich/Project|Project]]
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| - | !align="center"|[[Team:LMU-Munich/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:LMU-Munich/Modeling|Modeling]]
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| - | !align="center"|[[Team:LMU-Munich/Notebook|Notebook]]
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| - | !align="center"|[[Team:LMU-Munich/Safety|Safety]]
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| - | !align="center"|[[Team:LMU-Munich/Attributions|Attributions]]
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| - | ==<font color="#9933CC">'''Overall project'''</font>==
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| - | Metals and especially heavy metals are highly prescribed in concentrations in the drinking water ordinance. Qualifying and quantifying these by standard chemical methods is costly and complicated.
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| - | Bacteria sense metals in their surrounding in order to change their expression profile or react in order to adapt and accomodate to their environment.
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| - | Using these sensors from (mostly) bacteria we create biosensors by linking them to the expression of a reporter (e.g. green glowing by the green fluorescent protein GFP). To not only qualify but also to quantify the metals, it is also necessary to measure the output by given input (metal concentration) for each of these biosensors. Afterwards one can determine the metal concentration by measuring the output.
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| - | The quantification needs heavy high-tech machinery ... something not always given ... especially in free field. So a qualification of metals with a easy-to-see output is also needed.
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| - | In the end our team hopes to have not only a set of metallsensors for precise quantification of a group of (heavy) metalls, but also an outdoor kit for qualifying metalls in more remote areas. With these it might be more easy and cheaper to determine the content of metals in our drinking water.
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| - | == Project Details==
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| - | === Part 2 ===
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| - | === The Experiments ===
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| - | === Part 3 ===
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| - | == Results ==
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Project Abstract
Heavy metal ions of human origin contaminate waters worldwide and represent a major threat to human health, especially in lesser developed countries. The compliance with strict drinking water quality standards as a prerequisite to a healthy living requires qualitative and quantitative methods for monitoring metal ion concentration. Applying standard chemical methods is costly, complicated and sometimes requires high-tech machinery, which is often not available - especially where it's most urgently needed.
We therefore aim at creating a set of bacterial biosensors for some of the most toxic heavy metal ions found in drinking water, by fusing metal-responsive promoters under the control of transcriptional regulators with reporter genes such as GFP. The biosensors will be evaluated to qualitatively determine the metal ion specificity and subsequently quantitatively describing the concentration-dependent output of the reporters. Such a tool kit can be applied to quantify the metal ion content in water samples in an easier and cheaper way.
Detailed information can be found here!
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