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Team:LMU-Munich/Lab Notebook
From 2011.igem.org
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After weeks of hard work the last week of our lab work started. As we already prepared our BioBricks for sending them last week there was only the shipment left. So on Monday we said Good-bye and hope that they were going to have a nice journey to Boston. | After weeks of hard work the last week of our lab work started. As we already prepared our BioBricks for sending them last week there was only the shipment left. So on Monday we said Good-bye and hope that they were going to have a nice journey to Boston. | ||
So until Wednesday, the Wiki-freeze, are only a few tasks left: finishing the testing of our BioBricks BBa_K549001 and BBa_K549002 and of course to prepare the last thins for the european jamboree in Amsterdam. Making the presentation, the poster and of course ordering our team-sweaters. | So until Wednesday, the Wiki-freeze, are only a few tasks left: finishing the testing of our BioBricks BBa_K549001 and BBa_K549002 and of course to prepare the last thins for the european jamboree in Amsterdam. Making the presentation, the poster and of course ordering our team-sweaters. | ||
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=Protocols= | =Protocols= | ||
Revision as of 09:41, 20 September 2011
Week 1
This week we finally started the most important part of our project: the lab work. So of course we had first of all to setup the lab for our needs. Providing schottbottels, flasks, test glasses, ... This week also the first batch of primers arrived so we started the first PCRs.
The PCR for pnikA was done using the Phusion polymerase, the primers pnikA-E,N,X-for and pnikA-S-rev. The annealing temperature was set for 50°C. The template came from the gDNA of the Escherichia coli strain MG1655. (For more information abour the pnikA-system click here).
and prchA
Week 2
Still doing PCRs and start restriction digests.
Week 3
First Biobrick is ready!
Week 4
Week 5
Week 6
Week 7
Week 8
After weeks of hard work the last week of our lab work started. As we already prepared our BioBricks for sending them last week there was only the shipment left. So on Monday we said Good-bye and hope that they were going to have a nice journey to Boston. So until Wednesday, the Wiki-freeze, are only a few tasks left: finishing the testing of our BioBricks BBa_K549001 and BBa_K549002 and of course to prepare the last thins for the european jamboree in Amsterdam. Making the presentation, the poster and of course ordering our team-sweaters.
Protocols
Primer
pnikA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC |
pnikA-S-rev | CCGCTACTAGTAGACGATAAAAGACGCACAAGCC |
prcnA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca |
prcnA-S-rev | CCGCTACTAGTAcgcaccaagtaagatggcg |
luxCDrev | CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT |
PbrR-M,R-fwd | GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG |
PbrR-S,A-rev | AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG |
pbrRT-S,A-rev | agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC |
pbRMut-fwd | tcgtgcgggattctccagggactgtcggactgc |
pbrMut-rev | tccgacagtccctggagaatcccgcacgattgggc |
ppbrA-E,N,X-fwd | AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC |
ppbrA-S-rev | GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC |
luxCDfor | GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA |
luxBfor | ATGAAATTTGGATTGTATGAAATTTGGATTGT |
luxBrev | CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC |
luxEfor | GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA |
luxErev | CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA |
iscSfor | AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC |
icsSrev | agtcactagtattaaccggtctattaatgatgagcccattcg |
pabAfor | AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG |
pabArev | agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC |
aurFfor | AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC |
aurFrev | agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG |
ChrBAfor | AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC |
ChrBArev | agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG |
melAfor | gatcTCTAGAtgGCCGGCGCGTGGCTGGTCGGCAAGCCG |
melArev | gatcACCGGTGGCGGACACTATGGCTATTTCTAGC |
