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Team:LMU-Munich/Lab Notebook
From 2011.igem.org
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=Week 1= | =Week 1= | ||
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| + | This week we finally started the most important part of our project: the lab work. So of course we had first of all to setup the lab for our needs. Providing schottbottels, flasks, test glasses, ... This week also the first batch of primers arrived so we started the first PCRs. | ||
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| + | The PCR for | ||
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| + | and prchA | ||
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=Week 2= | =Week 2= | ||
Still doing PCRs and start restriction digests. | Still doing PCRs and start restriction digests. | ||
Revision as of 07:58, 20 September 2011
Week 1
This week we finally started the most important part of our project: the lab work. So of course we had first of all to setup the lab for our needs. Providing schottbottels, flasks, test glasses, ... This week also the first batch of primers arrived so we started the first PCRs.
The PCR for
and prchA
Week 2
Still doing PCRs and start restriction digests.
Week 3
First Biobrick is ready!
Week 4
Week 5
Week 6
Week 7
Week 8
Protocols
Primer
pnikA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGTTAAGCCTTGCGATCTGCACC |
pnikA-S-rev | CCGCTACTAGTAGACGATAAAAGACGCACAAGCC |
prcnA-E,N,X-fwd | GCAGAATTCGCGGCCGCTTCTAGAGacggattgtatgagacatggca |
prcnA-S-rev | CCGCTACTAGTAcgcaccaagtaagatggcg |
luxCDrev | CTGCAGCGGCCGCTACTAGTATTAAGACAGAGAAATTGCTTGAT |
PbrR-M,R-fwd | GCAGAATTCGCGGCCGCTTCTAGAGAAGAAGGAGATATACCATGAATATCCAGATCGGCGAG |
PbrR-S,A-rev | AGCCTGCAGCGGCCGCTACTAGTAttaCTAGTCGCTTGGATGGGCG |
pbrRT-S,A-rev | agtcactagtattaaccggttaTTACACCTGGGTAGATGGCC |
pbRMut-fwd | tcgtgcgggattctccagggactgtcggactgc |
pbrMut-rev | tccgacagtccctggagaatcccgcacgattgggc |
ppbrA-E,N,X-fwd | AGCCTGCAGCGGCCGCTACTAGTAGGTTGCGCGTCGCAACGGAAGC |
ppbrA-S-rev | GCAGAATTCGCGGCCGCTTCTAGAGCATGCGGTGCGCTTGGCAAGC |
luxCDfor | GAATTCCGCGGCCGCTTCTAGATGGAAAATGAATCAAAATA |
luxBfor | ATGAAATTTGGATTGTATGAAATTTGGATTGT |
luxBrev | CTGCAGCGGCCGCTACTAGTATTAGGTATATTCC |
luxEfor | GAATTCCGCGGCCGcttctagaATGTGACTGGGGTGAGTGA |
luxErev | CTGCAGCGGCCGCTACTAGTACTATCAAACGCTTCGGTTAA |
iscSfor | AgtcgccggcAAGAAGGAGATATACCATGTACGGAGTTTATAGAGC |
icsSrev | agtcactagtattaaccggtctattaatgatgagcccattcg |
pabAfor | AgtcgccggcAAGAAGGAGATATACCATGAAATTGCTATTAATTGATAATTATG |
pabArev | agtcactagtattaaccggtTTATTACACCACTTTCAAAAAATTATTTAAC |
aurFfor | AgtcgccggcAAGAAGGAGATATACCATGCCACGACACCGCGGGC |
aurFrev | agtcactagtattaaccggttaTCAACGCGGCGTGTGGGGCG |
ChrBAfor | AgtcgccggcAAGAAGGAGATATACCATGAACGCTCTCCCATCCTC |
ChrBArev | agtcactagtattaaccggttaTCAGTGATGCAACAACGGATAGG |
melAfor | gatcTCTAGAtgGCCGGCGCGTGGCTGGTCGGCAAGCCG |
melArev | gatcACCGGTGGCGGACACTATGGCTATTTCTAGC |
