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Team:Glasgow/iGEM Diary
From 2011.igem.org
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Want to know how life in the lab is going? Check out our weekly videos!<br/> | Want to know how life in the lab is going? Check out our weekly videos!<br/> | ||
| - | |||
<h1>Week 1 Summary</h1> | <h1>Week 1 Summary</h1> | ||
This week the video is presented by Michal Przydacz. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz>here</a> to find out more about Michal!<br/><br/> | This week the video is presented by Michal Przydacz. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Michal_Przydacz>here</a> to find out more about Michal!<br/><br/> | ||
| - | <iframe width="420" height="315" src="http://www.youtube.com/embed/0sEjIqsmJxY" frameborder="0" allowfullscreen></iframe> | + | <iframe width="420" height="315" src="http://www.youtube.com/embed/0sEjIqsmJxY" frameborder="0" allowfullscreen align=LEFT></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | Our new lab was set up<br/> | ||
| + | We created glycerol stocks of <i>E.coli</i> and <i>Pseudomonas</i> strains for future use<br/> | ||
| + | We had a meeting with <a href=http://www.gla.ac.uk:443/ibls/staff/staff.php?who=Ped|dQ>Dr Daniel Walker</a> who gave us some really useful information<br/> | ||
| + | We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them<br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
<h1>Week 2 Summary</h1> | <h1>Week 2 Summary</h1> | ||
This week the video is presented by Ellin Hillert. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert>here</a> to find out more about Ellin!<br/><br/> | This week the video is presented by Ellin Hillert. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Ellin_Hillert>here</a> to find out more about Ellin!<br/><br/> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/7wHpCv_hCOY" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/7wHpCv_hCOY" frameborder="0" allowfullscreen align=LEFT></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | We began the week with yet another transformation failure, resulting in our first successful transformants!<br/> | ||
| + | Our secret strain of <i>E.coli</i> arrived!<br/> | ||
| + | We each gave a presentation on different aspects of our project<br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | |||
<h1>Week 3 Summary</h1> | <h1>Week 3 Summary</h1> | ||
This week the video is presented by Andrew Landels. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels>here</a> to find out more about Andrew!<br /><br/> | This week the video is presented by Andrew Landels. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Andrew_Landels>here</a> to find out more about Andrew!<br /><br/> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/MhnMF-s4DRo" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/MhnMF-s4DRo" frameborder="0" allowfullscreen align=LEFT></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | We now have successful transformations of all our biobricks!<br/> | ||
| + | Our secret strain of <i>E.coli</i> forms really nice biofilms!<br/> | ||
| + | We had our very first Wiki Whaaaaaam!<br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | |||
| + | |||
<h1>Week 4 Summary</h1> | <h1>Week 4 Summary</h1> | ||
This week the video is presented by Pietro Ridone. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone>here</a> to find out more about Pietro!<br/><br/> | This week the video is presented by Pietro Ridone. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Pietro_Ridone>here</a> to find out more about Pietro!<br/><br/> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/BpCmEpKz-h4" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/BpCmEpKz-h4" frameborder="0" allowfullscreen align=LEFT></iframe></center> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | Work to gather data on biofilm growth and dispersal continues. <br/> | ||
| + | Primers for biobricks arrived<br/> | ||
| + | The guys have given us a sneak preview of the Norwich presentation<br/> | ||
| + | We have acquired our own Flouresence Microscope...<br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | |||
| + | |||
<h1>Week 5 Summary</h1> | <h1>Week 5 Summary</h1> | ||
This week the video is presented by Chris Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Chris_Wood>here</a> to find out more about Chris!<br/><br/> | This week the video is presented by Chris Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Chris_Wood>here</a> to find out more about Chris!<br/><br/> | ||
| - | <iframe width="420" height="315" src="http://www.youtube.com/embed/rs6vdCWG8yU" frameborder="0" allowfullscreen></iframe> | + | <iframe width="420" height="315" src="http://www.youtube.com/embed/rs6vdCWG8yU" frameborder="0" allowfullscreen align=left></iframe></center> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | The guys have returned from Norwich with some great insights<br/> | ||
| + | Everyone is excited to incorporate human practices into the project in a new way<br/> | ||
| + | We now have a plan for Modelling<br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
<h1>Week 6 Summary</h1> | <h1>Week 6 Summary</h1> | ||
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<iframe width="560" height="315" src="http://www.youtube.com/embed/6S6KtfYjfg8" frameborder="0" allowfullscreen></iframe> | <iframe width="560" height="315" src="http://www.youtube.com/embed/6S6KtfYjfg8" frameborder="0" allowfullscreen></iframe> | ||
| + | |||
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | We started ligating our constructs<br/> | ||
| + | PCR was carried out on our novel biobricks<br/> | ||
| + | Our secret <i>E.coli</i> strain was successfully transformed with RFP! <br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | |||
| + | |||
<h1>Week 7 Summary</h1> | <h1>Week 7 Summary</h1> | ||
This week the video is presented by Emma Campbell. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell>here</a> to find out more about Emma!<br/><br/> | This week the video is presented by Emma Campbell. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Emma_Campbell>here</a> to find out more about Emma!<br/><br/> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/epBvEuJJtds" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/epBvEuJJtds" frameborder="0" allowfullscreen align=left></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | Edinburgh sent us some biobricks to work with<br/> | ||
| + | We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis<br/> | ||
| + | Our poster for the Open Day is looking great! | ||
| + | Amsterdam is booked - everyone is really excited!<br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
<h1>Week 8 Summary</h1> | <h1>Week 8 Summary</h1> | ||
This week the video is presented by Stewart O'Neill. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Stewart_O%27Neill>here</a/> to find out more about Stewart!<br/><br/> | This week the video is presented by Stewart O'Neill. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Stewart_O%27Neill>here</a/> to find out more about Stewart!<br/><br/> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/ZiJpsuw36nM" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/ZiJpsuw36nM" frameborder="0" allowfullscreen align=left></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | Work on our light responsive constructs and Edinburgh biobricks is continuing<br/> | ||
| + | An EnvZ negative strain of <i>E.coli</i> arrived from the Registry<br/> | ||
| + | Modelling is going really well<br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | |||
<h1>Week 9 Summary</h1> | <h1>Week 9 Summary</h1> | ||
This week the video is presented by Scott Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Scott_Wood>here</a> to find out more about Scott!<br/><br> | This week the video is presented by Scott Wood. Click <a href=https://2011.igem.org/Team:Glasgow/Team/Scott_Wood>here</a> to find out more about Scott!<br/><br> | ||
| - | <iframe width="560" height="315" src="http://www.youtube.com/embed/ePeOcq4zEKY" frameborder="0" allowfullscreen></iframe> | + | <iframe width="560" height="315" src="http://www.youtube.com/embed/ePeOcq4zEKY" frameborder="0" allowfullscreen align=left></iframe> |
| + | <br/><br/> <u>This week:</u><br/> | ||
| + | PCR and Site Directed Mutagenesis is continuing on our novel biobricks<br/> | ||
| + | Our work with the EnvZ negative cells is ongoing<br/> | ||
| + | Modelling looks great - we now have some really great graphs for our dispersal biobricks! <br/><br/> | ||
| + | <br/><br/><br/><br/><br/><br/><br/><br/> | ||
| + | <center> | ||
Revision as of 00:53, 20 September 2011
Want to know how life in the lab is going? Check out our weekly videos!
Week 1 Summary
This week the video is presented by Michal Przydacz. Click here to find out more about Michal!This week:
Our new lab was set up
We created glycerol stocks of E.coli and Pseudomonas strains for future use
We had a meeting with Dr Daniel Walker who gave us some really useful information
We chose the biobricks we would need (various Promoters, Ribosome Binding Sites and Terminators)and began transforming them
Week 2 Summary
This week the video is presented by Ellin Hillert. Click here to find out more about Ellin!This week:
We began the week with yet another transformation failure, resulting in our first successful transformants!
Our secret strain of E.coli arrived!
We each gave a presentation on different aspects of our project
Week 3 Summary
This week the video is presented by Andrew Landels. Click here to find out more about Andrew!This week:
We now have successful transformations of all our biobricks!
Our secret strain of E.coli forms really nice biofilms!
We had our very first Wiki Whaaaaaam!
Week 4 Summary
This week the video is presented by Pietro Ridone. Click here to find out more about Pietro!This week:
Work to gather data on biofilm growth and dispersal continues.
Primers for biobricks arrived
The guys have given us a sneak preview of the Norwich presentation
We have acquired our own Flouresence Microscope...
Week 5 Summary
This week the video is presented by Chris Wood. Click here to find out more about Chris!This week:
The guys have returned from Norwich with some great insights
Everyone is excited to incorporate human practices into the project in a new way
We now have a plan for Modelling
Week 6 Summary
This week the video is presented by Hannah Ralph. Click here to find out more about Hannah!This week:
We started ligating our constructs
PCR was carried out on our novel biobricks
Our secret E.coli strain was successfully transformed with RFP!
Week 7 Summary
This week the video is presented by Emma Campbell. Click here to find out more about Emma!This week:
Edinburgh sent us some biobricks to work with
We realised our novel biobricks have illegal restriction sites, so ordered primers for Site Directed Mutagenesis
Our poster for the Open Day is looking great! Amsterdam is booked - everyone is really excited!
Week 8 Summary
This week the video is presented by Stewart O'Neill. Click here to find out more about Stewart!This week:
Work on our light responsive constructs and Edinburgh biobricks is continuing
An EnvZ negative strain of E.coli arrived from the Registry
Modelling is going really well
Week 9 Summary
This week the video is presented by Scott Wood. Click here to find out more about Scott!This week:
PCR and Site Directed Mutagenesis is continuing on our novel biobricks
Our work with the EnvZ negative cells is ongoing
Modelling looks great - we now have some really great graphs for our dispersal biobricks!
