Team:DTU-Denmark-2

From 2011.igem.org

Revision as of 22:04, 19 September 2011

• Home
• About us
  • The Team
  • Supervisors
  • Gallery
• The Project
  • Abstract
  • Introduction
  • The Plug 'n' Play
    • Assembly system
    • Customization
  • Other assembly systems
  • Achievements
  • Attributions
• Results
  • Background
  • Characterization
  • Proof of concept
    • Mammalian Cells
    • Fungi
  • Data page
  • Submitted Biobricks
  • Collaboration
• Safety
  • Safety
  • Safety rules
• Notebook
  • Lab notes
  • Protocols
• Our Partners
  • Sponsors
  • Advisors
  • Acknowledgements

Making Synthetic Biology easier We have designed a novel standardized assembly system, called “Plug 'n' Play with DNA” where ready to use biological parts can directly be gathered without the use of restriction enzymes and ligases. This will make cloning faster and assembly of expression vectors possible within a few hours. We have created a library of standardized biological parts for mammalian cells and Aspergillus niger ready to plug 'n' play. You can find more information about how this standard works here. Creating a reporter system As proof of concept we in very short time created a reporter system that can be used for anything from monitoring gene expression to determination of protein localization. We chose to express and/or localize fluorescent proteins in the model organism Aspergillus nidulans and the mammalian cell line U-2 OS with success. Flexibility Standardization entails rigidity. We acknowledge this and have therefore written a guide that allows the user to customize the system. In this way proteins can be assembled seamless, multiple mutations can be introduced in one round of cloning etc., the only thing setting a limit is your creativity. Applications Here you can learn more about the numerous applications of the Plug’n’Play with DNA assembly standard and the situations where the use of this system is especially advantageous. Data Page You can find an overview of our project on our Data Page. This is also the place where you can find our favorite submitted biobricks and the biobricks we characterized during the summer. Achievements Designing a novel assembly standard and creating a reporter system for Aspergillus nidulans and mammalian cells are our main achievements. You can read more about our accomplishments on this page. The Team We are five girls working on this two and a half month project with support from our three supervisors. You can learn more about us and how we started an iGEM team on our Team Page.

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