Team:Wageningen UR/Project/DevicesSetup

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(→Customary fluidic device designed by Team Wageningen UR to measure oscillations)
(→Customary fluidic device designed by Team Wageningen UR to measure oscillations)
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=== Setup ===
=== Setup ===
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==== Chosing which platform to use for measuring oscillations ====
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[[File:Hasty_device_WUR.png|200px]]  .  [[File:Legend_device_WUR.png|180px]][[File:Micro-sieve_device_WUR.png|300px|right]]
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[[File:Micro-dish1_device_WUR.png|350px]] [[File:Flushingoutofwells_WUR.gif|280px|right]]
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[[File:Micro-dish2_device_WUR.png|300px|center]]
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[[Team:Wageningen_UR/Project/ModelingProj1#Modeling_synchronized_oscillations| back to top]]
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==== Controlling the flow rates ====
==== Controlling the flow rates ====
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In order to be able to observe oscillations, the device was designed in such a way that it was possible to place it under a fluorescence microscope for measuring GFP.  
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As already mentioned in the [[Team:Wageningen_UR/Project/Devices| design]] section of the device, the chamber was constructed in such a way that it was possible to place it under a fluorescence microscope for measuring GFP.  

Revision as of 16:01, 19 September 2011

Building a Synchronized Oscillatory System

Customary fluidic device designed by Team Wageningen UR to measure oscillations

Setup

Chosing which platform to use for measuring oscillations

Hasty device WUR.png . Legend device WUR.png
Micro-sieve device WUR.png



Micro-dish1 device WUR.png
280px


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Micro-dish2 device WUR.png

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Controlling the flow rates

The main concern for the setup of the device was to be able to gain control over the flow rate. According to Bernoulli's principle, the velocity of a fluid can be influenced by varying the height of the medium bottle. This approach was also used in the paper cited above. Figure X. shows the corresponding setup and the applying equations.

Scheme setup1 WUR.png
Setup1 WUR.JPG


Equations bernoulli WUR.png

Fig.X: Setup of the device using Bernoulli's principle to control the velocity of the fluid.

However, as mentioned on the modeling page, the dimensions of our fluidic device did not allow the aimed for precise control over the flow rate. This was tested both by calculating some theoretical values applicable for our device and running pilot experiments with water. Furthermore the obtained flow rates were also much faster than the flow rates in which oscillations were to be expected. This was solved by expanding our setup to incorporate the use of a syringe pump which controls the inflow. Figure X+1 shows the new setup.

Scheme setup2 WUR.png

Fig.X+1: Setup of the device using a pump to control the velocity of the fluid.


As already mentioned in the design section of the device, the chamber was constructed in such a way that it was possible to place it under a fluorescence microscope for measuring GFP.


Closeup device WUR.JPG
Setup WUR.jpg


Top: Devices under the microscope

Right: Entire setup of the system around the fluorescence microscope

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Controlling cell growth

During the measurements, the bacteria were bottom fed as seen in the scheme in figure X+2. This allowed the measurements to be taken continuously for various hours.

Bottom feed WUR.png

Fig.X+2: Applying bottom feeding to keep the cells in the wells alive



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