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<a name="fig01"><h2>Figure 1</h2></a><br> | <a name="fig01"><h2>Figure 1</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig01.jpg" border="0"><br><br> |
Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L | Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K<sub>1</sub>= terminator, K<sub>2</sub>= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L | ||
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<a name="fig02"><h2>Figure 2</h2></a><br> | <a name="fig02"><h2>Figure 2</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig02.jpg" border="0"><br><br> |
Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator. | Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator. | ||
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<a name="fig03"><h2>Figure 3</h2></a><br> | <a name="fig03"><h2>Figure 3</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig03.jpg" border="0"><br><br> |
Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor. | Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor. | ||
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<a name="fig04"><h2>Figure 4</h2></a><br> | <a name="fig04"><h2>Figure 4</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig04.jpg" border="0"><br><br> |
Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, <i>H</i><sub>2</sub>= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 3<sub>1</sub>= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 3<sub>1</sub>= Mnt repressor, <i>H</i><sub>1</sub>= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI. | Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, <i>H</i><sub>2</sub>= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 3<sub>1</sub>= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 3<sub>1</sub>= Mnt repressor, <i>H</i><sub>1</sub>= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI. | ||
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<a name="fig05"><h2>Figure 5</h2></a><br> | <a name="fig05"><h2>Figure 5</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig05.jpg" border="0"><br><br> |
Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor. | Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor. | ||
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<a name="fig06"><h2>Figure 6</h2></a><br> | <a name="fig06"><h2>Figure 6</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig06.jpg" border="0"><br><br> |
Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI). | Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI). | ||
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<a name="fig07"><h2>Figure 7</h2></a><br> | <a name="fig07"><h2>Figure 7</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig07.jpg" border="0"><br><br> |
Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI). | Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI). | ||
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<a name="fig08"><h2>Figure 8</h2></a><br> | <a name="fig08"><h2>Figure 8</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig08.jpg" border="0"><br><br> |
Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI. | Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI. | ||
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<a name="fig09"><h2>Figure 9</h2></a><br> | <a name="fig09"><h2>Figure 9</h2></a><br> | ||
| - | <img src="http:// | + | <img src="http://homes.esat.kuleuven.be/~igemwiki/images/notebook/wetlab_fig09.jpg" border="0"><br><br> |
<br><br><a href="javascript: history.go(-1)"><i>← Click here to go back</i></a><br><br><br> | <br><br><a href="javascript: history.go(-1)"><i>← Click here to go back</i></a><br><br><br> | ||
Latest revision as of 11:51, 19 September 2011
FIGURES
Figure 1
Miniprep 13/07: L, A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, D= Ribolock-Coilicin E2 Dnase, E= ribolock3c, F= ribokey3d, G= GFP Generator, H= CrtEBI, I= CI repressor, J= ribosome binding site, K1= terminator, K2= pconst-RBS-MelA, Z= CspA Promoter, 1= luxI, 2= luxR , 3= Mnt repressor, 4= OmpA, 5= TEV cleavage site, L
← Click here to go back
Figure 2
Miniprep 15/07: labelling is the same as in Fig.1 except for K= pconst-RBS-MelA and Y= terminator.
← Click here to go back
Figure 3
Restriction 18/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, I= CI repressor, G= GFP generator, H= CrtEBI, 3= Mnt repressor.
← Click here to go back
Figure 4
Restriction 20/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, H2= RBS-CrtEBI, K= terminator, Z= cspA Promoter, 31= Mnt repressor were all cut with EcoRI and SpeI. V= luxI, K= terminator, 31= Mnt repressor, H1= RBS-CrtEBI and G= GFP generator were all cut with XbaI and PstI.
← Click here to go back
Figure 5
Miniprep 21/07: A= pLux-CI promotor, B= pLac/Mnt Hybrid Promoter, G= GFP generator, H= CrtEBI, I= CI repressor, U= constitutive promotor, V= LuxI, W= BAD promotor, X= Ribokey3d, 3= Mnt repressor.
← Click here to go back
Figure 6
Restriction 25/07: U= Constitutieve promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI) K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).
← Click here to go back
Figure 7
Results of gel purification: U= Constitutive promotor (PstI and SpeI), W= BAD promoter (PstI and SpeI), Z= CspA promotor (PstI and SpeI), K= Terminator (EcoRI and XbaI), X= ribokey3d (EcoRI and XbaI).
← Click here to go back
Figure 8
Restriction 28/07: G= GFP generator, T= LuxR, V= LuxI.
← Click here to go back
Figure 9
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