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Team:Kyoto/Notebook
From 2011.igem.org
(→Week3: Monday 15th - Sunday 21th August) |
(→Week3: Monday 15th - Sunday 21th August) |
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Left primer: tctagaggtgacgtttatgcaacga<br/> | Left primer: tctagaggtgacgtttatgcaacga<br/> | ||
Right primer: actagtacacacaagctgtgaatcactc<br/> | Right primer: actagtacacacaagctgtgaatcactc<br/> | ||
| - | annealing temperature was 55 | + | annealing temperature was 55 degrees.<br/> |
~~photo~~<br/> | ~~photo~~<br/> | ||
lane 1,2 &7,8: DNA ladder(λDNA digested Hindlll,100bp), lane 3,4: glnG1,glnG2, lane 5,6: glnL1,glnL2<br/> | lane 1,2 &7,8: DNA ladder(λDNA digested Hindlll,100bp), lane 3,4: glnG1,glnG2, lane 5,6: glnL1,glnL2<br/> | ||
| Line 111: | Line 111: | ||
glnL1: 137.4ng/ul<br/> | glnL1: 137.4ng/ul<br/> | ||
glnL2: 124.2ng/ul<br/> | glnL2: 124.2ng/ul<br/> | ||
| - | |||
| - | |||
'''Friday'''<br/> | '''Friday'''<br/> | ||
| + | Nutritional Signal(Shimosaka)<br/> | ||
| + | Restriction of glnG1 and glnG2<br/> | ||
| + | Cut them with Xbal and Spel for 2 hours at 37 degrees.<br/> | ||
| + | Then, gel extraction of digested.<br/> | ||
'''Saturday'''<br/> | '''Saturday'''<br/> | ||
Revision as of 08:14, 19 September 2011
Lab Note
Week1: Monday 1st - Sunday 7th August
Monday
行った実験名：
使った試薬名、容量：
用いた機械：
行った人：
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week2: Monday 8th - Sunday 14th August
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week3: Monday 15th - Sunday 21th August
Monday
Tuesday
Wednesday
Thursday
Nutritional Signal(Sugiura,Shimosaka & Okumura)
PCR Amplification of glnL and glnG from gDNA of E.coli
--Primers--
glnL
Left primer: tctagaggagactgctttatggcaac
Right primer: actagtaggaactatcgtcatcgactac
glnG
Left primer: tctagaggtgacgtttatgcaacga
Right primer: actagtacacacaagctgtgaatcactc
annealing temperature was 55 degrees.
~~photo~~
lane 1,2 &7,8: DNA ladder(λDNA digested Hindlll,100bp), lane 3,4: glnG1,glnG2, lane 5,6: glnL1,glnL2
After purification, the concentration of DNA are
glnG1: 127.8ng/ul
glnG2: 118.1ng/ul
glnL1: 137.4ng/ul
glnL2: 124.2ng/ul
Friday
Nutritional Signal(Shimosaka)
Restriction of glnG1 and glnG2
Cut them with Xbal and Spel for 2 hours at 37 degrees.
Then, gel extraction of digested.
Saturday
Sunday
Week4: Monday 22th - Sunday 28th August
Monday
Tuesday
Wednesday
Thursday
Luminescence(Kusaba, Terada, Hara):ハエの走行性実験① ♂、紫外線×2回、緑×2回 ♀、紫外線×2回、緑×2回
Friday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤外線×2回 ♀、赤外線×2回
Nutritional Signal(Hashiya):
Transformation of bellow parts.
1,4-17M:BBa_K325909(lux operon)
2,1-12M:BBa_E0240
3,2-17F:BBa_120260(low copy vector)
Saturday
Luminescence(Kusaba):ハエの走行性実験① ♂、赤×2回、青×2回 ♀、赤×2回、青×2回
Sunday
Week5: Monday 29th August - Sunday 4th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week6: Monday 5th September - Sunday 11th September
Monday
Tuesday
Luminescence(Kusaba, Hara):ハエの走行性実験②（②は改良版） ♂、緑×2回 ♀、青×1回
Wednesday
Luminescence(Hara):ハエの走行性実験② ♂、青×2回 ♀、緑×2回、青×1回
Thursday
Luminescence(Hara):ハエの走行性実験② ♂、紫外線×3回 ♀、紫外線×3回
Friday
Saturday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、青×2回、赤×2回 ♀、青×2回、赤×2回
Sunday
Luminescence(Kusaba, Hara):ハエの走行性実験② ♂、紫外線×2回、
Week7: Monday 12th September - Sunday 18th September
Monday
Luminescence:大腸菌の形質転換(Hashiya) ハエの走行性実験②(Kusaba, Hara)
Tuesday
Luminescence:大腸菌はじめて光る。しかし光量は少ない。
Wednesday
Thursday
Friday
Saturday
Sunday
Week8: Monday 19th September - Sunday 25th September
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Week9: Monday 26th September - Sunday 2nd October
Monday
Tuesday
Wednesday
Thursday
Friday
Saturday
Sunday
Protocol
Medium for drosophila
[http://www.biol.se.tmu.ac.jp/fly/www/standard-medium.html Medium for drosophila] Materials Methods - water : 500mL
- dry yeast : 20g
- corn flour : 45g
- glucose : 50g
- agarose : 3.5~5g
- propionic acid : 1.5mL
- 10% p-hydroxybenzoate in 70% Eternol : 5g
- Stir dry yeast and agarose with about two-thirds of water. Then, autoclave it.
- Stir corn flour and glucose with the remaining water.
- Stir 1 and 2, then autoclave it again.
- after autoclave, add propionic acid and 10% p-hydroxybenzoate in 70% Eternol into it.■
