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Team:BU Wellesley Software/Notebook/AlbertoNotebook
From 2011.igem.org
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B. Pbad | B. Pbad | ||
| - | Using the IGEM plate from spring 2010, Pbad promoter biobrick was obtained and transformed into ecoli bacteria. The bacteria were then plated onto agar-based LB+Ampicillin growth medium. Lots of cultures were found on the next day after the overnight transformation. Plasmid prep was | + | Using the IGEM plate from spring 2010, Pbad promoter biobrick was obtained and transformed into ecoli bacteria. The bacteria were then plated onto agar-based LB+Ampicillin growth medium. Lots of cultures were found on the next day after the overnight transformation. However, they appeared to be either dark or light. Plasmid prep was done for each of the color type and each tube was incubated for 12 hours. On the next morning, the tip in each and the resulting plasmids were quantified with nanodrop. Their respective concentrations were 10.0 ng/uL and 8.3 ng/uL. They were found to be relatively free from contaminants (based on the 260/280 and 260/230 values). None of the samples appeared on the gel after electrophoresis. |
Revision as of 20:35, 13 June 2011
'2011 IGEM WetLab Notebook - Weekly Log'
6/6/11-10/6/11
A. BFP2
Plasmid prep were created from the BFP2 culture plate and incubated for approximately 16 hours for plasmid amplification. The resulting plasmids were isolated by miniprep and quantified with nanodrop. The DNA concentration in the two samples are 16.5 ng/uL and 14.9 ng/uL, respectively. While such values are below the normally acceptable range of 20 and above, we still decided to run electrophoresis on them. No band is detected in the gel.
B. Pbad
Using the IGEM plate from spring 2010, Pbad promoter biobrick was obtained and transformed into ecoli bacteria. The bacteria were then plated onto agar-based LB+Ampicillin growth medium. Lots of cultures were found on the next day after the overnight transformation. However, they appeared to be either dark or light. Plasmid prep was done for each of the color type and each tube was incubated for 12 hours. On the next morning, the tip in each and the resulting plasmids were quantified with nanodrop. Their respective concentrations were 10.0 ng/uL and 8.3 ng/uL. They were found to be relatively free from contaminants (based on the 260/280 and 260/230 values). None of the samples appeared on the gel after electrophoresis.
