Team:DTU-Denmark-2

From 2011.igem.org

(Difference between revisions)
Line 62: Line 62:
     padding-bottom:20px;
     padding-bottom:20px;
     padding-top:20px;
     padding-top:20px;
 +
    background-color:#F7BE81;
      
      
-
    background-color:#F7BE81
+
  clear:both;
}
}
Line 71: Line 72:
     padding-bottom:10px;
     padding-bottom:10px;
     padding-top:10px;
     padding-top:10px;
-
     
+
    width: 170.9px;
-
     background-color:#BEF781
+
    height: 170.9px;
 +
     background-color:#BEF781;
 +
    float:left;
 +
    margin:5px;
}
}
  div.blue {
  div.blue {
Line 79: Line 83:
     padding-bottom:10px;
     padding-bottom:10px;
     padding-top:10px;
     padding-top:10px;
-
       background-color:#A9E2F3
+
    width: 170.9px;
 +
    height: 170.9px;
 +
       background-color:#A9E2F3;
 +
float:left;
 +
margin:5px;
}
}
  div.yellow {
  div.yellow {
-
padding-right: 10px;  
+
    padding-right: 10px;  
     padding-left: 10px;
     padding-left: 10px;
     padding-bottom:10px;
     padding-bottom:10px;
     padding-top:10px;
     padding-top:10px;
-
     background-color:#F3F781
+
    width: 170.9px;
 +
    height: 170.9px;
 +
    float:left;
 +
    margin:5px;
 +
     background-color:#F3F781;
}
}
Line 131: Line 143:
<div class="green">
<div class="green">
-
<font color="#990000" face="arial" size="4" >
+
<font color="#000000" face="arial" size="3" >
<b>Assembly Standard</b><br>
<b>Assembly Standard</b><br>
-
<br>
+
 
-
<br>
+
-
<br>
+
-
<br>
+
</div>
</div>
<div class="blue">
<div class="blue">
-
<font color="#990000" face="arial" size="4" >
+
<font color="#000000" face="arial" size="3" >
<b>Mammalian cells</b><br>
<b>Mammalian cells</b><br>
-
<br>
+
 
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
</div>
</div>
<div class="yellow">
<div class="yellow">
-
<ui>
+
 
-
<font color="#990000" face="arial" size="4" >
+
<font color="#000000" face="arial" size="3" >
<b>Filamentous Fungi</b><br>
<b>Filamentous Fungi</b><br>
-
<br>
+
 
-
<br>
+
-
<br>
+
-
<br>
+
-
<br>
+
</div>
</div>
<br>
<br>
Line 163: Line 164:
<div class="orange">
<div class="orange">
<ui>
<ui>
-
<font color="#990000" face="arial" size="6" >
+
<font color="#000000" face="arial" size="5" >
<b>Achievements</b><br>
<b>Achievements</b><br>
</font>
</font>
-
<font color="#000000" face="arial" size="3" >
+
<font color="#000000" face="arial" size="2" >
<br>
<br>
<li> We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.</li>
<li> We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.</li>
Line 257: Line 258:
  </td>
  </td>
</table>
</table>
-
<p align="justify">
 
-
<div class="orange">
 
-
<ui>
 
-
<font color="#990000" face="arial" size="6" >
 
-
<b>Achievements</b><br>
 
-
</font>
 
-
<font color="#000000" face="arial" size="3" >
 
-
<br><b>
 
-
<li> We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.</li>
 
-
<li> We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts.</li>
 
-
<li> We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy.</li>
 
-
<li> We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy.</li>
 
-
<li> We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team.</li>
 
-
<li> We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays.</li>
 
-
</b>
 
-
</font>
 
-
</ui>
 
-
</div>
 
-
</p>
 
-
<br>
 
-
 
-
 
<br>
<br>
<br>
<br>

Revision as of 20:29, 18 September 2011



Revolutionizing Synthetic Biology


The DTU-Denmark 2 iGEM team has designed a novel standardized assembly system, called “Plug 'n' Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. This will make synthetic biology faster and assembly of an expression vector possible within a few hours. We have created a library of standardized biological parts for mammalian cells and fungi ready to plug 'n' play.

Assembly Standard
Mammalian cells
Filamentous Fungi




Achievements

  • We have achieved to create a novel assembly standard system for BioBrick called Plug'n Play with DNA.
  • We submitted 49 new parts and 21 plasmids to the Registry of Standard Biological Parts.
  • We demonstrated the function of our system in the mammalian cell line U2-OS by fluorescence and confocal microscopy.
  • We demonstrated the function of our system in filamentous fungi Aspergilli by fluorescence and microscopy.
  • We demonstrated the function of our system in E. coli by helping the Copenhagen iGEM team.
  • We achieved to characterized two new fungal promoter by use of ß-galactosidase assays and Bradford assays.












  • Sponsored by

     



       

           

    Comments or questions to the team? Please