Team:Freiburg/Description

From 2011.igem.org

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(Blue light receptor)
(Blue light receptor)
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In the light state the LovTAP binds to the operator DNA and inhibits the gene expression.  To avoid this inhibition, we cloned a Not-Gate after the LovTAP.  Not-Gate is like an inverter, because it changes the input to its opposite. The Not-Gate consists of a tetracycline repressor and a TetR repressible promotor . In the light state the LovTAP binds to operators DNA and inhibits the expression of tetracycline repressor  and the TetR promotor is active. In the dark state the tryptophan repressor is expressed and inhibits the expression of the protein of interest.
In the light state the LovTAP binds to the operator DNA and inhibits the gene expression.  To avoid this inhibition, we cloned a Not-Gate after the LovTAP.  Not-Gate is like an inverter, because it changes the input to its opposite. The Not-Gate consists of a tetracycline repressor and a TetR repressible promotor . In the light state the LovTAP binds to operators DNA and inhibits the expression of tetracycline repressor  and the TetR promotor is active. In the dark state the tryptophan repressor is expressed and inhibits the expression of the protein of interest.
[[File:Freiburg-2011-bluelight1.jpg|thumb|600px]]
[[File:Freiburg-2011-bluelight1.jpg|thumb|600px]]
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==<span style="color:red;">Red light receptor</span>==
==<span style="color:red;">Red light receptor</span>==

Revision as of 20:02, 18 September 2011


This is the wiki page
of the Freiburger student
team competing for iGEM 2011.
Thank you for your interest!