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Team:DTU-Denmark/Gel preparation and gel electrophoresis
From 2011.igem.org
(Difference between revisions)
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== Gel preparation (1% gel) (100 ml of the buffer) == | == Gel preparation (1% gel) (100 ml of the buffer) == | ||
| + | Ingredients: | ||
<ol style="list-style-type:lower-alpha"> | <ol style="list-style-type:lower-alpha"> | ||
<li value="a">100 ml of 1x TBE buffer.</li> | <li value="a">100 ml of 1x TBE buffer.</li> | ||
| Line 6: | Line 7: | ||
<li value="d">Assembled gel container.</li> | <li value="d">Assembled gel container.</li> | ||
</ol> | </ol> | ||
| - | + | Procedure | |
# Mix buffer with agarose and heat in microwave until the solution is clear | # Mix buffer with agarose and heat in microwave until the solution is clear | ||
# Add 10μl of ethidium bromide (10mg/ml). | # Add 10μl of ethidium bromide (10mg/ml). | ||
Revision as of 14:13, 18 September 2011
Gel preparation (1% gel) (100 ml of the buffer)
Ingredients:
- 100 ml of 1x TBE buffer.
- 10 μl of ethidium bomide (10 mg/ml).
- 1 g of agarose.
- Assembled gel container.
Procedure
- Mix buffer with agarose and heat in microwave until the solution is clear
- Add 10μl of ethidium bromide (10mg/ml).
- Pour solution to the gel container and leave it to solidify (30-45 min).
- Place gel container in the electrophoresis machine, remove combs and cover with the TBE buffer.
Gel electrophoresis:
- 2μl of DNA sample.
- 3 μl of distilled water.
- 1μl of 6x loading dye.
- 4.2 μl of Gene Ruler DNA ladder mix from Fermentas.
It is recommended to use 7V for each cm of the gel length and to run the gel for 45 min.
