Team:DTU-Denmark-2

From 2011.igem.org

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<ui>
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<li> We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi. </li>
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<li> We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence. </li>
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<li> We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy. </li>
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<li>We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/submitted biobricks"> List </a>.</li>
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<li>We applied for a gold medal by fulfilling the <a hret="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievement"> requirements listed</a> by iGEM 2011. </li>
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<b> Why Plug 'n' Play with DNA?</b><br>
<b> Why Plug 'n' Play with DNA?</b><br>
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<ui>
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<li> We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi. </li>
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<li> We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence. </li>
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<li> We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy. </li>
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<li>We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts <a href="https://2011.igem.org/Team:DTU-Denmark-2/results/submitted biobricks"> List </a>.</li>
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<li>We applied for a gold medal by fulfilling the <a hret="https://2011.igem.org/Team:DTU-Denmark-2/Project/achievement"> requirements listed</a> by iGEM 2011. </li>
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</ui>
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Revision as of 10:31, 18 September 2011



Our mission

insert text here!

  • We achieved to design a standardized cloning system, called Plug'n Play with DNA, for both mammalian cells and fungi.
  • We characterized proof of concept BioBrick with ß-galactosidase assays, Bradford assay and fluorescence.
  • We demonstrated the function of our system i mammalian cells by fluorescence and confocal microscopy.
  • We submitted 49 parts and 21 plasmids to the Registry of Standard Biological Parts List .
  • We applied for a gold medal by fulfilling the requirements listed by iGEM 2011.


    • Why Plug 'n' Play with DNA?

    We have developed a standardized assembly system, called “Plug’n’Play with DNA”, where any biological parts can be gathered without use of restriction enzymes and ligases. The method applies long complementary overhangs on the PCR product as well as the destination vector. These overhangs are custom made and can anneal to each other to form a stable hybridization product. This means that the parts in the form of pre-produced PCR-products are directly mixed with a vector, which makes assembly of an expression vector possible within a few hours. All the parts in the form of pre-produced PCR-products will be distributed in microtiter plates directly ready for cloning. The simple and easy use of the system was demonstrated by developing a reporter targeting system for the mammalian cell line, U2OS, where GFP was targeted to the peroxisomes. In addition, application of our system was demonstrated in the fungus Aspergillus nidulans.


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