Team:NTNU Trondheim/Stress Sensor

From 2011.igem.org

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!Biobrick
!Biobrick
!Part number
!Part number
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|rrnB P1
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|BBa_K639002
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|LacI with RBS
|LacI with RBS

Revision as of 08:29, 17 September 2011



Stress Sensor (rrnB+LacI+pLac+mCherry)

RrnB+LacI+pLac+mCherry rosa.png


The stress sensor is our main construct. It is made from the following biobricks:

Plasmid map of the complete construct. Promoters are shown in blue, and genes as inside arrows. Only single restriction sites are shown.
Biobrick Part number
rrnB P1 BBa_K639002
LacI with RBS BBa_J24679
Terminator BBa_B0015
pLac BBa_R0011
mCherry with RBS and terminator BBa_J06702

The promotor is a variant of BBa_K112118, that we have made ourselves using PCR, and the PCR product was directly used as insert and connected with the rest of the construct. The final construct was cut with BstBI to verify that the rrnB P1 had been insertet. The stress sensor has a total length of 2653 bp, and is located inside the plasmid pSB1A2, carrying ampicillin resistance. We have put the stress sensor through several tests (see characterization)

Link to partsregistry: [http://partsregistry.org/Part:BBa_K639003 BBa_K639003]


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