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Team:Michigan/Protocols
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[[Media: electroporate.pdf | Electroporation of electrocompetent cells]] | [[Media: electroporate.pdf | Electroporation of electrocompetent cells]] | ||
| - | [[Media: competent.pdf | Production of standard competent cells]] | + | [[Media: competent.pdf | Production of standard/QT competent cells]] |
[[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | [[Media: quick_transformation.pdf | Quick Transformation (QT) of standard competent cells]] | ||
| - | + | [[Media: heat_shock.pdf | Heat shock transformation of standard competent cells.]] | |
'''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | '''Enzyme usage''' ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations. | ||
Revision as of 23:15, 10 June 2011
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Protocols
Basic protocols ::: These protocols are necessary for all lab operations.
Gels and electrophoresis ::: These protocols contain information on the production and usage of gels.
Transformation ::: These protocols contain information on the production, usage, and transformation of competent cells.
Production of electrocompetent cells
Electroporation of electrocompetent cells
Production of standard/QT competent cells
Quick Transformation (QT) of standard competent cells
Heat shock transformation of standard competent cells.
Enzyme usage ::: These protocols contain information on digestion, ligation, and specific information on enzymatic operations.
